Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1972 Sep;2(3):159–163. doi: 10.1128/aac.2.3.159

3-Deazauridine: Inhibition of Ribonucleic Acid Virus-Induced Cytopathogenic Effects In Vitro

William M Shannon 1, Gussie Arnett 1, Frank M Schabel Jr 1
PMCID: PMC444284  PMID: 4799550

Abstract

3-Deazauridine, a synthetic analogue of uridine and a potential antitumor agent, was found to possess antiviral activity against a number of ribonucleic acid-containing animal viruses in mammalian cell culture. Inhibition of virus-induced cytopathogenic effects by 3-deazauridine was observed in cells infected with rhinovirus types 1A and 13, coxsackievirus type A21, and PR-8 influenza virus, but not in cells infected with poliovirus type 2 or echovirus type 12. The extracellular yield of progeny influenza virus was found to be significantly reduced in titer in 3-deazauridine-treated Madin-Darby canine kidney cell cultures at 12 to 24 hr after infection. Although the precise biochemical mechanism of action of this antimetabolite's antiviral activity is not known, the compound does not appear to exert a direct virucidal effect on the influenza virion itself.

Full text

PDF
159

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BABLANIAN R., EGGERS H. J., TAMM I. STUDIES ON THE MECHANISM OF POLIOVIRUS-INDUCED CELL DAMAGE. I. THE RELATION BETWEEN POLIOVIRUS,-INDUCED METABOLIC AND MORPHOLOGICAL ALTERATIONS IN CULTURED CELLS. Virology. 1965 May;26:100–113. doi: 10.1016/0042-6822(65)90030-9. [DOI] [PubMed] [Google Scholar]
  2. BALTIMORE D., FRANKLIN R. M. A NEW RIBONUCLEIC ACID POLYMERASE APPEARING AFTER MENGOVIRUS INFECTION OF L-CELLS. J Biol Chem. 1963 Oct;238:3395–3400. [PubMed] [Google Scholar]
  3. BARRY R. D., IVES D. R., CRUICKSHANK J. G. Participation of deoxyribonucleic acid in the multiplication of influenza virus. Nature. 1962 Jun 23;194:1139–1140. doi: 10.1038/1941139a0. [DOI] [PubMed] [Google Scholar]
  4. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  5. EAGLE H. Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc Soc Exp Biol Med. 1955 Jul;89(3):362–364. doi: 10.3181/00379727-89-21811. [DOI] [PubMed] [Google Scholar]
  6. Ehrlich J., Sloan B. J., Miller F. A., Machamer H. E. Searching for antiviral materials from microbial fermentations. Ann N Y Acad Sci. 1965 Jul 30;130(1):5–16. doi: 10.1111/j.1749-6632.1965.tb12533.x. [DOI] [PubMed] [Google Scholar]
  7. FRANKLIN R. M., BALTIMORE D. Patterns of macromolecular synthesis in normal and virus-infected mammalian cells. Cold Spring Harb Symp Quant Biol. 1962;27:175–198. doi: 10.1101/sqb.1962.027.001.019. [DOI] [PubMed] [Google Scholar]
  8. Gaush C. R., Hard W. L., Smith T. F. Characterization of an established line of canine kidney cells (MDCK). Proc Soc Exp Biol Med. 1966 Jul;122(3):931–935. doi: 10.3181/00379727-122-31293. [DOI] [PubMed] [Google Scholar]
  9. Gaush C. R., Smith T. F. Replication and plaque assay of influenza virus in an established line of canine kidney cells. Appl Microbiol. 1968 Apr;16(4):588–594. doi: 10.1128/am.16.4.588-594.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Green M. Oncogenic viruses. Annu Rev Biochem. 1970;39:701–756. doi: 10.1146/annurev.bi.39.070170.003413. [DOI] [PubMed] [Google Scholar]
  11. HULL R. N., CHERRY W. R., TRITCH O. J. Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research. J Exp Med. 1962 May 1;115:903–918. doi: 10.1084/jem.115.5.903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Khare G. P., Sidwell R. W., Huffman J. H., Tolman R. L., Robins R. K. Inhibition of RNA virus replication in vitro by 3-deazacytidine and 3-deazauridine. Proc Soc Exp Biol Med. 1972 Jul;140(3):880–884. doi: 10.3181/00379727-140-36571. [DOI] [PubMed] [Google Scholar]
  13. RIGHTSEL W. A., SCHULTZ P., MUETHING D., MCLEAN I. W., Jr Use of vinyl plastic containers in tissue cultures for virus assays. J Immunol. 1956 Jun;76(6):464–474. [PubMed] [Google Scholar]
  14. ROTT R., SCHOLTISSEK C. EINFLUSS VON ACTINOMYCIN AUF DIE VERMEHRUNG VON MYXOVIREN. Z Naturforsch B. 1964 Apr;19:316–323. doi: 10.1007/BF01191316. [DOI] [PubMed] [Google Scholar]
  15. Rott R., Scholtissek C. Specific inhibition of influenza replication by alpha-amanitin. Nature. 1970 Oct 3;228(5266):56–56. doi: 10.1038/228056a0. [DOI] [PubMed] [Google Scholar]
  16. Schlom J., Spiegelman S., Moore D. H. Detection of high-molecular-weight RNA in particles from human milk. Science. 1972 Feb 4;175(4021):542–544. doi: 10.1126/science.175.4021.542. [DOI] [PubMed] [Google Scholar]
  17. Sidwell R. W., Arnett G., Dixon G. J., Schabel F. M., Jr Purine analogs as potential anticytomegalovirus agents. Proc Soc Exp Biol Med. 1969 Sep;131(4):1223–1230. doi: 10.3181/00379727-131-34075. [DOI] [PubMed] [Google Scholar]
  18. WHITE D. O., DAY H. M., BATCHELDER E. J., CHEYNE I. M., WANSBROUGH A. J. DELAY IN THE MULTIPLICATION OF INFLUENZA VIRUS. Virology. 1965 Feb;25:289–302. doi: 10.1016/0042-6822(65)90207-2. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES