Skip to main content
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1972 Nov;2(5):340–343. doi: 10.1128/aac.2.5.340

Inhibition of Growth of T-Strain Mycoplasmas by Hydroxamic Acids and by Aurothiomalate

Denys K Ford 1
PMCID: PMC444317  PMID: 4207956

Abstract

Sorbyl-, benzoyl-, and 3-amino-benzoyl hydroxamic acids inhibited the development of an alkaline pH by T-strain cultures grown in broth containing 0.05% urea and phenol red. The specificity of this urease inhibition was demonstrated by the inhibition, by 10−4m sorbyl-hydroxamic acid, of the release of 14CO2 from 14C-urea by washed T-strain mycoplasmas in 4 hr of incubation. Sorbyl-, benzoyl-, and 3-amino-benzoyl hydroxamic acids at a concentration of 10−3m markedly inhibited the multiplication of T-strain 354 during 18 hr of incubation; this inhibition was not corrected by thymidine at a concentration of 500 μg per ml. Aurothiomalate was 20 times more inhibitory to Mycoplasma hominis DC-63 than to T-strain 354; equivalent inhibitory concentrations were 50 μg per ml for M. hominis and 1,200 μg per ml for T strains.

Full text

PDF
340

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Csonka G. W., Spitzer R. J. Lincomycin, non-gonococcal urethritis, and mycoplasmata. Br J Vener Dis. 1969 Mar;45(1):52–54. doi: 10.1136/sti.45.1.52. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davidson M., Thomas L. Effect of gold on Mycoplasma. Antimicrob Agents Chemother (Bethesda) 1966;6:312–315. [PubMed] [Google Scholar]
  3. FISHBEIN W. N., CARBONE P. P. UREASE CATALYSIS. II. INHIBITION OF THE ENZYME BY HYDROXYUREA, HYDROXYLAMINE, AND ACETOHYDROXAMIC ACID. J Biol Chem. 1965 Jun;240:2407–2414. [PubMed] [Google Scholar]
  4. Ford D. K. CULTURE OF HUMAN GENITAL "T-STRAIN" PLEUROPNEUMONIA-LIKE ORGANISMS. J Bacteriol. 1962 Nov;84(5):1028–1034. doi: 10.1128/jb.84.5.1028-1034.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ford D. K., MacDonald J. Influence of urea on the growth of T-strain mycoplasmas. J Bacteriol. 1967 May;93(5):1509–1512. doi: 10.1128/jb.93.5.1509-1512.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ford D. K., McCandlish K. L., Gronlund A. F. Metabolism of 14C-urea by T-strain mycoplasma. J Bacteriol. 1970 May;102(2):605–606. doi: 10.1128/jb.102.2.605-606.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gale G. R., Atkins L. M. Inhibition of urease by hydroxamic acids. Arch Int Pharmacodyn Ther. 1969 Aug;180(2):289–298. [PubMed] [Google Scholar]
  8. Gale G. R., Smith A. B., Walker E. M., Jr Selective inhibition by sorbylhydroxamic acid of deoxyribonucleic acid synthesis in Ehrlich ascites tumor cells. Cancer Res. 1970 Jan;30(1):24–29. [PubMed] [Google Scholar]
  9. KOBASHI K., HASE J., UEHARA K. Specific inhibition of urease by hydroxamic acids. Biochim Biophys Acta. 1962 Dec 4;65:380–383. doi: 10.1016/0006-3002(62)91067-3. [DOI] [PubMed] [Google Scholar]
  10. SHEPARD M. C. T-Form colonies of pleuropneumonialike organisms. J Bacteriol. 1956 Mar;71(3):362–369. doi: 10.1128/jb.71.3.362-369.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sabin A. B., Warren J. The Curative Effect of Certain Gold Compounds on Experimental Proliferative, Chronic Arthritis in Mice. J Bacteriol. 1940 Dec;40(6):823–856. doi: 10.1128/jb.40.6.823-856.1940. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shepard M. C., Lunceford C. D. Occurrence of urease in T strains of Mycoplasma. J Bacteriol. 1967 May;93(5):1513–1520. doi: 10.1128/jb.93.5.1513-1520.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES