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. 1972 Dec;2(6):438–441. doi: 10.1128/aac.2.6.438

Relationship Between the Uptake of Isoniazid and Its Action on In Vivo Mycolic Acid Synthesis in Mycobacterium tuberculosis

Lynn Wang 1,2,1, Kuni Takayama 1,2
PMCID: PMC444336  PMID: 4207759

Abstract

A direct relationship was established between the rate of uptake of isoniazid and the action of this drug on in vivo mycolic acid synthesis in Mycobacterium tuberculosis H37Ra. The rate of uptake of isoniazid increased linearly with its external concentration and appeared to reach a maximal value of 52 pmoles per hr per 109 cells at an external concentration of about 13 μm. Correspondingly, the rate of inhibition of mycolic acid synthesis increased with the rise in the rate of uptake of the drug. A 50% inhibition of mycolic acid synthesis occurred when the uptake of isoniazid reached 5.2 pmoles per 109 cells. Calculations showed that this level of drug uptake represents an internal cellular concentration of 9 μm. These results show clearly that the action of isoniazid on the mycolate synthetase system of M. tuberculosis is rapid and that this enzyme system is highly sensitive to the drug.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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