Abstract
An oxidation product of indole-3-acetic acid, 3-methyleneoxindole, is highly reactive with free sulfhydryl groups. It has been shown to be a potent bactericidal agent in actively growing cultures. The bactericidal action of 3-methyleneoxindole was found to be independent of protein or deoxyribonucleic acid synthesis but to require the capacity for ribonucleic acid synthesis. The reactions underlying the bactericidal effect of 3-methyleneoxindole are not unique to it among compounds which can bind free SH groups, since N-ethylmaleimide appeared to have a similar mode of action.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BOREK E., RYAN A., ROCKENBACH J. Nucleic acid metabolism in relation to the lysogenic phenomenon. J Bacteriol. 1955 Apr;69(4):460–467. doi: 10.1128/jb.69.4.460-467.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edlin G., Broda P. Physiology and genetics of the "ribonucleic acid control" locus in escherichia coli. Bacteriol Rev. 1968 Sep;32(3):206–226. doi: 10.1128/br.32.3.206-226.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FUKUYAMA T. T., MOYED H. S. INHIBITION OF CELL GROWTH BY PHOTOOXIDATION PRODUCTS OF INDOLE-3-ACETIC ACID. J Biol Chem. 1964 Jul;239:2392–2397. [PubMed] [Google Scholar]
- GALE E. F., FOLKES J. P. The assimilation of amino-acids by bacteria. XV. Actions of antibiotics on nucleic acid and protein synthesis in Staphylococcus aureus. Biochem J. 1953 Feb;53(3):493–498. doi: 10.1042/bj0530493. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rosenkranz H. S., Garro A. J., Levy J. A., Carr H. S. Studies with hydroxyurea. I. The reversible inhibition of bacterial DNA synthesis and the effect of hydroxyurea on the bactericidal action of streptomycin. Biochim Biophys Acta. 1966 Mar 21;114(3):501–515. [PubMed] [Google Scholar]
- STILL C. C., FUKUYAMA T. T., MOYED H. S. INHIBITORY OXIDATION PRODUCTS OF INDOLE-3-ACETIC ACID. MECHANISM OF ACTION AND ROUTE OF DETOXIFICATION. J Biol Chem. 1965 Jun;240:2612–2618. [PubMed] [Google Scholar]
- Tuli V., Moyed H. S. Desensitization of regulatory enzymes by a metabolite of plant auxin. J Biol Chem. 1966 Oct 10;241(19):4564–4566. [PubMed] [Google Scholar]
- Tuli V., Moyed H. S. Inhibitory oxidation products of indole-3-acetic Acid: 3-hydroxymethyloxindole and 3-methyleneoxindole as plant metabolites. Plant Physiol. 1967 Mar;42(3):425–430. doi: 10.1104/pp.42.3.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tuli V., Moyed H. S. The role of 3-methyleneoxindole in auxin action. J Biol Chem. 1969 Sep 25;244(18):4916–4920. [PubMed] [Google Scholar]
- WISSEMAN C. L., Jr, SMADEL J. E., HAHN F. E., HOPPS H. E. Mode of action of chloramphenicol. I. Action of chloramphenicol on assimilation of ammonia and on synthesis of proteins and nucleic acids in Escherichia coli. J Bacteriol. 1954 Jun;67(6):662–673. doi: 10.1128/jb.67.6.662-673.1954. [DOI] [PMC free article] [PubMed] [Google Scholar]