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. 1973 Feb;3(2):299–305. doi: 10.1128/aac.3.2.299

Mechanism of Chloramphenicol Resistance in Staphylococci: Characterization and Hybridization of Variants of Chloramphenicol Acetyltransferase

L C Sands 1,2, W V Shaw 1,2
PMCID: PMC444403  PMID: 4790593

Abstract

Fifty clinical isolates of chloramphenicol-resistant staphylococci from diverse sources were screened for the presence of chloramphenicol acetyltransferase (CAT) and were found to contain the inducible chloramphenicol-inactivating enzyme in each case. Polyacrylamide disc gel electrophoresis revealed four distinct types of CAT of which three were purified to a state of homogeneity. Each purified CAT preparation was shown to exist as a tetrameric protein with a native molecular weight of 80,000 and an identical subunit size of 20,000. All four staphylococcal types of CAT exhibited identical catalytic and immunological properties, but they possessed variable sensitivity to heat denaturation and to inhibition by mercuric ion. Each of the three purified variants of staphylococcal CAT was capable of undergoing reversible denaturation in 6 m guanidine hydrochloride. In vitro hybridization was successful between pairs of each of the three purified staphylococcal enzymes. Only one heteromeric (hybrid) species was observed in addition to the parental types rather than the three predicted from the known quaternary structure of staphylococcal CAT.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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