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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1994 Aug 2;91(16):7563–7567. doi: 10.1073/pnas.91.16.7563

Localization of a putative tumor suppressor gene by using homozygous deletions in melanomas.

J Weaver-Feldhaus 1, N A Gruis 1, S Neuhausen 1, D Le Paslier 1, E Stockert 1, M H Skolnick 1, A Kamb 1
PMCID: PMC44442  PMID: 8052620

Abstract

The p21 region of human chromosome 9 is thought to contain a gene (MLM) involved in genetic susceptibility to melanoma and a gene or genes that influence progression of certain other tumors. Genomic clones that span a large region in 9p21 surrounding the presumptive tumor suppressor gene(s) have been isolated. A set of sequence-tagged sites in this region has been developed. By using these markers and others previously reported, the 9p21 region has been studied by physical mapping in 84 melanoma cell lines. A putative tumor suppressor gene, perhaps MLM itself, has been localized to a region of less than 40 kb that lies proximal (centromeric) to the alpha-interferon gene cluster.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bergman W., Watson P., de Jong J., Lynch H. T., Fusaro R. M. Systemic cancer and the FAMMM syndrome. Br J Cancer. 1990 Jun;61(6):932–936. doi: 10.1038/bjc.1990.209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cannon-Albright L. A., Goldgar D. E., Meyer L. J., Lewis C. M., Anderson D. E., Fountain J. W., Hegi M. E., Wiseman R. W., Petty E. M., Bale A. E. Assignment of a locus for familial melanoma, MLM, to chromosome 9p13-p22. Science. 1992 Nov 13;258(5085):1148–1152. doi: 10.1126/science.1439824. [DOI] [PubMed] [Google Scholar]
  4. Cohen D., Chumakov I., Weissenbach J. A first-generation physical map of the human genome. Nature. 1993 Dec 16;366(6456):698–701. doi: 10.1038/366698a0. [DOI] [PubMed] [Google Scholar]
  5. Diaz M. O., Ziemin S., Le Beau M. M., Pitha P., Smith S. D., Chilcote R. R., Rowley J. D. Homozygous deletion of the alpha- and beta 1-interferon genes in human leukemia and derived cell lines. Proc Natl Acad Sci U S A. 1988 Jul;85(14):5259–5263. doi: 10.1073/pnas.85.14.5259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elder D. E. Human melanocytic neoplasms and their etiologic relationship with sunlight. J Invest Dermatol. 1989 May;92(5 Suppl):297S–303S. doi: 10.1111/1523-1747.ep13076732. [DOI] [PubMed] [Google Scholar]
  7. Fountain J. W., Karayiorgou M., Ernstoff M. S., Kirkwood J. M., Vlock D. R., Titus-Ernstoff L., Bouchard B., Vijayasaradhi S., Houghton A. N., Lahti J. Homozygous deletions within human chromosome band 9p21 in melanoma. Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10557–10561. doi: 10.1073/pnas.89.21.10557. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gruis N. A., Sandkuijl L. A., Weber J. L., van der Zee A., Borgstein A. M., Bergman W., Frants R. R. Linkage analysis in Dutch familial atypical multiple mole-melanoma (FAMMM) syndrome families. Effect of naevus count. Melanoma Res. 1993 Aug;3(4):271–277. [PubMed] [Google Scholar]
  9. Kwiatkowski D. J., Diaz M. O. Dinucleotide repeat polymorphism at the IFNA locus (9p22). Hum Mol Genet. 1992 Nov;1(8):658–658. doi: 10.1093/hmg/1.8.658-a. [DOI] [PubMed] [Google Scholar]
  10. Lukeis R., Irving L., Garson M., Hasthorpe S. Cytogenetics of non-small cell lung cancer: analysis of consistent non-random abnormalities. Genes Chromosomes Cancer. 1990 Jul;2(2):116–124. doi: 10.1002/gcc.2870020207. [DOI] [PubMed] [Google Scholar]
  11. Middleton P. G., Prince R. A., Williamson I. K., Taylor P. R., Reid M. M., Jackson G. H., Katz F., Chessells J. M., Proctor S. J. Alpha interferon gene deletions in adults, children and infants with acute lymphoblastic leukemia. Leukemia. 1991 Aug;5(8):680–682. [PubMed] [Google Scholar]
  12. Nancarrow D. J., Mann G. J., Holland E. A., Walker G. J., Beaton S. C., Walters M. K., Luxford C., Palmer J. M., Donald J. A., Weber J. L. Confirmation of chromosome 9p linkage in familial melanoma. Am J Hum Genet. 1993 Oct;53(4):936–942. [PMC free article] [PubMed] [Google Scholar]
  13. Olopade O. I., Buchhagen D. L., Malik K., Sherman J., Nobori T., Bader S., Nau M. M., Gazdar A. F., Minna J. D., Diaz M. O. Homozygous loss of the interferon genes defines the critical region on 9p that is deleted in lung cancers. Cancer Res. 1993 May 15;53(10 Suppl):2410–2415. [PubMed] [Google Scholar]
  14. Olopade O. I., Jenkins R. B., Ransom D. T., Malik K., Pomykala H., Nobori T., Cowan J. M., Rowley J. D., Diaz M. O. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 1992 May 1;52(9):2523–2529. [PubMed] [Google Scholar]

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