Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1973 Apr;3(4):517–522. doi: 10.1128/aac.3.4.517

Suppression by 1-β-d-Ribofuranosyl-1,2,4-Triazole-3-Carboxamide (Virazole, ICN 1229) of Influenza Virus-Induced Infections in Mice

Gyaneshwar P Khare 1, Robert W Sidwell 1, J T Witkowski 1, Lionel N Simon 1, Roland K Robins 1
PMCID: PMC444445  PMID: 4790606

Abstract

1-β-d-Ribofuranosyl-1,2,4-triazole-3-carboxamide (Virazole) was found to possess significant antiviral activity against aerosol-induced or intranasally induced influenza A0, A2, and B virus infections in mice. Significant protection was achieved by both oral and intraperitoneal routes of administration. Depending upon the level of virus infection, antiviral activity was best observed at the daily dose of 75 mg/kg. The efficacy of the compound was evidenced by an increase in survivor number, prolongation of mean survival time, suppression of lung consolidation, or decrease in hemagglutinin titer in the infected lung samples. The therapeutic value of this synthetic triazole nucleoside was evident as noted by a significant increase in survivor number even if the treatment was started as late as 24 h after infection with an aerosol of influenza A2 virus.

Full text

PDF
517

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAVIES W. L., GRUNERT R. R., HAFF R. F., MCGAHEN J. W., NEUMAYER E. M., PAULSHOCK M., WATTS J. C., WOOD T. R., HERMANN E. C., HOFFMANN C. E. ANTIVIRAL ACTIVITY OF 1-ADAMANTANAMINE (AMANTADINE). Science. 1964 May 15;144(3620):862–863. doi: 10.1126/science.144.3620.862. [DOI] [PubMed] [Google Scholar]
  2. Hill D. A., Baron S., Chanock R. M. The effect of an interferon inducer on influenza virus. Bull World Health Organ. 1969;41(3):689–693. [PMC free article] [PubMed] [Google Scholar]
  3. Huffman J. H., Sidwell R. W., Khare G. P., Witkowski J. T., Allen L. B., Robins R. K. In vitro effect of 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide (virazole, ICN 1229) on deoxyribonucleic acid and ribonucleic acid viruses. Antimicrob Agents Chemother. 1973 Feb;3(2):235–241. doi: 10.1128/aac.3.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Khare G. P., Sidwell R. W., Huffman J. H., Tolman R. L., Robins R. K. Inhibition of RNA virus replication in vitro by 3-deazacytidine and 3-deazauridine. Proc Soc Exp Biol Med. 1972 Jul;140(3):880–884. doi: 10.3181/00379727-140-36571. [DOI] [PubMed] [Google Scholar]
  5. MIDDLEBROOK G. An apparatus for airborne infection of mice. Proc Soc Exp Biol Med. 1952 May;80(1):105–110. doi: 10.3181/00379727-80-19538. [DOI] [PubMed] [Google Scholar]
  6. Sidwell R. W., Huffman J. H., Khare G. P., Allen L. B., Witkowski J. T., Robins R. K. Broad-spectrum antiviral activity of Virazole: 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide. Science. 1972 Aug 25;177(4050):705–706. doi: 10.1126/science.177.4050.705. [DOI] [PubMed] [Google Scholar]
  7. Witkowski J. T., Robins R. K., Sidwell R. W., Simon L. N. Design, synthesis, and broad spectrum antiviral activity of 1- -D-ribofuranosyl-1,2,4-triazole-3-carboxamide and related nucleosides. J Med Chem. 1972 Nov;15(11):1150–1154. doi: 10.1021/jm00281a014. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES