Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1973 Jul;4(1):1–5. doi: 10.1128/aac.4.1.1

Lividomycin Resistance in Staphylococci by Enzymatic Phosphorylation

Fujio Kobayashi 1,2, Tomoyuki Koshi 1,2, Junji Eda 1,2, Yoko Yoshimura 1,2, Susumu Mitsuhashi 1,2
PMCID: PMC444495  PMID: 4364938

Abstract

Enzymatic inactivation of lividomycin (LV) was attempted with nine clinical isolates of staphylococci including LV-susceptible and -resistant strains. LV inactivation and the incorporation into LV of 32P from γ-32P-adenosine triphosphate were demonstrated in the presence of cell-free extracts from LV-resistant strains but not from LV-susceptible ones. The enzyme was purified approximately 82-fold from a resistant Staphylococcus aureus strain by means of ammonium sulfate fractionation and column chromatography. Some properties of the partially purified LV-phosphorylating enzyme were quite similar to those of an enzyme from Escherichia coli carrying an R factor conferring LV resistance, and the phosphorylated product of the drug was also found to be identical with that produced by E. coli carrying an R factor, i.e., 5″-phosphoryl-LV.

Full text

PDF
1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Davies J. E., Rownd R. Transmissible multiple drug resistance in Enterobacteriaceae. Science. 1972 May 19;176(4036):758–768. doi: 10.1126/science.176.4036.758. [DOI] [PubMed] [Google Scholar]
  2. Doi O., Miyamoto M., Tanaka N., Umezawa H. Inactivation and phosphorylation of kanamycin by drug-resistant Staphylococcus aureus. Appl Microbiol. 1968 Sep;16(9):1282–1284. doi: 10.1128/am.16.9.1282-1284.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HASHIMOTO H., KONO K., MITSUHASHI S. ELIMINATION OF PENICILLIN RESISTANCE OF STAPHYLOCOCCUS AUREUS BY TREATMENT WITH ACRIFLAVINE. J Bacteriol. 1964 Jul;88:261–262. doi: 10.1128/jb.88.1.261-262.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Inoue M., Oshima H., Okubo T., Mitsuhashi S. Isolation of the rec mutants in Staphylococcus aureus. J Bacteriol. 1972 Dec;112(3):1169–1176. doi: 10.1128/jb.112.3.1169-1176.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kawabe H., Mitsuhashi S. Inactivation of dihydrostreptomycin by Staphylococcus aureus. Jpn J Microbiol. 1971 Nov;15(6):545–548. doi: 10.1111/j.1348-0421.1971.tb00618.x. [DOI] [PubMed] [Google Scholar]
  6. Kobayashi F., Nagoya T., Yoshimura Y., Kaneko K., Ogata S. I. Studies on new antibiotic lividomycins. V. In vitro and in vivo antimicrobial activity of lividomycin A. J Antibiot (Tokyo) 1972 Feb;25(2):128–136. [PubMed] [Google Scholar]
  7. Kobayashi F., Yamaguchi M., Mitsuhashi S. Activity of lividomycin against Pseudomonas aeruginosa: its inactivation by phosphorylation induced by resistant strains. Antimicrob Agents Chemother. 1972 Jan;1(1):17–21. doi: 10.1128/aac.1.1.17. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kobayashi F., Yamaguchi M., Mitsuhashi S. Inactivation of dihydrostreptomycin by Pseudomonas aeruginosa. Jpn J Microbiol. 1971 Jul;15(4):381–382. doi: 10.1111/j.1348-0421.1971.tb00596.x. [DOI] [PubMed] [Google Scholar]
  9. Kobayashi F., Yamaguchi M., Mitsuhashi S. Phosphorylated inactivation of aminoglycosidic antibiotics by Pseudomonas aeruginosa. Jpn J Microbiol. 1971 May;15(3):265–272. doi: 10.1111/j.1348-0421.1971.tb00578.x. [DOI] [PubMed] [Google Scholar]
  10. Kondo S., Yamamoto H., Naganawa H., Umezawa H. Isolation and characterization of lividomycin A inactivated by Pseudomonas aeruginosa and Escherichia coli carrying R factor. J Antibiot (Tokyo) 1972 Aug;25(8):483–484. doi: 10.7164/antibiotics.25.483. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. MITSUHASHI S., MORIMURA M., KONO K., OSHIMA H. ELIMINATION OF DRUG RESISTANCE OF STAPHYLOCOCCUS AUREUS BY TREATMENT WITH ACRIFLAVINE. J Bacteriol. 1963 Jul;86:162–164. doi: 10.1128/jb.86.1.162-164.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. NOVICK R. P. ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS. J Gen Microbiol. 1963 Oct;33:121–136. doi: 10.1099/00221287-33-1-121. [DOI] [PubMed] [Google Scholar]
  14. Ozanne B., Benveniste R., Tipper D., Davies J. Aminoglycoside antibiotics: inactivation by phosphorylation in Escherichia coli carrying R factors. J Bacteriol. 1969 Nov;100(2):1144–1146. doi: 10.1128/jb.100.2.1144-1146.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Takasawa S., Utahara R., Okanishi M., Maeda K., Umezawa H. Studies on adenylylstreptomycin, a product of streptomycin inactivation by E. coli carrying the R-factor. J Antibiot (Tokyo) 1968 Aug;21(8):477–484. doi: 10.7164/antibiotics.21.477. [DOI] [PubMed] [Google Scholar]
  16. Umezawa H., Okanishi M., Kondo S., Hamana K., Utahara R., Maeda K., Mitsuhashi S. Phosphorylative inactivation of aminoglycosidic antibiotics by Escherichia coli carrying R factor. Science. 1967 Sep 29;157(3796):1559–1561. [PubMed] [Google Scholar]
  17. Yamada T., Tipper D., Davies J. Enzymatic inactivation of streptomycin by R factor-resistant Escherichia coli. Nature. 1968 Jul 20;219(5151):288–291. doi: 10.1038/219288a0. [DOI] [PubMed] [Google Scholar]
  18. Yamaguchi M., Kobayashi F., Mitsuhashi S. Antibacterial activity of lividomycin toward R factor-resistant strains of Escherichia coli. Antimicrob Agents Chemother. 1972 Feb;1(2):139–142. doi: 10.1128/aac.1.2.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yamaguchi M., Koshi T., Kobayashi F., Mitsuhashi S. Phosphorylation of lividomycin by Escherichia coli carrying an R factor. Antimicrob Agents Chemother. 1972 Sep;2(3):142–146. doi: 10.1128/aac.2.3.142. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES