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Fluorescence recovery after photobleaching (FRAP) curves recorded for (a) a fluid bilayer on a PCysMA brush formed after full incubation; (b) a double bilayer adsorbed on a PCysMA brush (dry thickness 5–8 nm) following incubation at 50 °C for 1 h; and (c) a single bilayer formed after 48 h of incubation at 20 °C for the sample shown in (b). The vesicles comprised 25% DOTAP, 75% POPC, and 0.5 mol % Texas red in 10 mM phosphate buffer at pH 7.
