Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1973 Jul;4(1):44–48. doi: 10.1128/aac.4.1.44

Synthesis of Vaccinia Virus Polypeptides in the Presence of Isatin-β-Thiosemicarbazone

Ehud Katz 1, Eva Margalith 1, Bela Winer 1, Natan Goldblum 1
PMCID: PMC444502  PMID: 4791481

Abstract

Isatin-β-thiosemicarbazone (IBT) at a concentration of 14 μM inhibited the multiplication of vaccinia virus in HeLa cells. For the first 3 h after infection, viral deoxyribonucleic acid (DNA) was synthesized in the presence of IBT at the same rate as in the control culture; the replication rate declined at a later stage. The DNA failed to be coated with proteins and to become resistant to deoxyribonuclease unless IBT was removed. “Early” and “late” viral polypeptides were formed in the presence of IBT, as revealed by polyacrylamide gel electrophoresis. The formation from precursor of a core polypeptide, a reaction blocked by rifampin, was not affected by IBT. Therefore, it is suggested that a maturation step later than the one blocked by rifampin is involved in the inhibition of vaccinia virus by IBT.

Full text

PDF
44

Images in this article

47Image
on p.47
47Image
on p.47
47Image
on p.47

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appleyard G., Hume V. B., Westwood J. C. The effect of thiosemicarbazones on the growth of rabbit pox virus in tissue culture. Ann N Y Acad Sci. 1965 Jul 30;130(1):92–104. doi: 10.1111/j.1749-6632.1965.tb12543.x. [DOI] [PubMed] [Google Scholar]
  2. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  3. EASTERBROOK K. B. Interference with the maturation of vaccinia virus by isatin beta-thiosemicarbazone. Virology. 1962 Jun;17:245–251. doi: 10.1016/0042-6822(62)90114-9. [DOI] [PubMed] [Google Scholar]
  4. Fairbanks G., Jr, Levinthal C., Reeder R. H. Analysis of C14-labeled proteins by disc electrophoresis. Biochem Biophys Res Commun. 1965 Aug 16;20(4):393–399. doi: 10.1016/0006-291x(65)90589-9. [DOI] [PubMed] [Google Scholar]
  5. Grimley P. M., Rosenblum E. N., Mims S. J., Moss B. Interruption by Rifampin of an early stage in vaccinia virus morphogenesis: accumulation of membranes which are precursors of virus envelopes. J Virol. 1970 Oct;6(4):519–533. doi: 10.1128/jvi.6.4.519-533.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JOKLIK W. K., BECKER Y. THE REPLICATION AND COATING OF VACCINIA DNA. J Mol Biol. 1964 Dec;10:452–474. doi: 10.1016/s0022-2836(64)80066-8. [DOI] [PubMed] [Google Scholar]
  7. Katz E., Moss B. Formation of a vaccinia virus structural polypeptide from a higher molecular weight precursor: inhibition by rifampicin. Proc Natl Acad Sci U S A. 1970 Jul;66(3):677–684. doi: 10.1073/pnas.66.3.677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Katz E., Moss B. Vaccinia virus structural polypeptide derived from a high-molecular-weight precursor: formation and integration into virus particles. J Virol. 1970 Dec;6(6):717–726. doi: 10.1128/jvi.6.6.717-726.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Moss B. Inhibition of HeLa cell protein synthesis by the vaccinia virion. J Virol. 1968 Oct;2(10):1028–1037. doi: 10.1128/jvi.2.10.1028-1037.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moss B., Rosenblum E. N., Katz E., Grimley P. M. Rifampicin: a specific inhibitor of vaccinia virus assembly. Nature. 1969 Dec 27;224(5226):1280–1284. doi: 10.1038/2241280a0. [DOI] [PubMed] [Google Scholar]
  11. Moss B., Salzman N. P. Sequential protein synthesis following vaccinia virus infection. J Virol. 1968 Oct;2(10):1016–1027. doi: 10.1128/jvi.2.10.1016-1027.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. SALZMAN N. P. The rate of formation of vaccinia deoxyribonucleic acid and vaccinia virus. Virology. 1960 Jan;10:150–152. doi: 10.1016/0042-6822(60)90015-5. [DOI] [PubMed] [Google Scholar]
  13. SHATKIN A. J. ACTINOMYCIN D AND VACCINIA VIRUS INFECTION OF HELA CELLS. Nature. 1963 Jul 27;199:357–358. doi: 10.1038/199357a0. [DOI] [PubMed] [Google Scholar]
  14. Salzman N. P., Sebring E. D. Sequential formation of vaccinia virus proteins and viral deoxyribonucleic acid replication. J Virol. 1967 Feb;1(1):16–23. doi: 10.1128/jvi.1.1.16-23.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Woodson B., Joklik W. K. The inhibition of vaccinia virus multiplication by isatin-beta-thiosemicarbazone. Proc Natl Acad Sci U S A. 1965 Sep;54(3):946–953. doi: 10.1073/pnas.54.3.946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Zylber E. A., Penman S. The effect of high ionic strength on monomers, polyribosomes, and puromycin-treated polyribosomes. Biochim Biophys Acta. 1970 Mar 19;204(1):221–229. doi: 10.1016/0005-2787(70)90505-8. [DOI] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES