Abstract
The 1-methylisatin derivative of isatin thiosemicarbazone was previously shown to inhibit granulocyte-macrophage colony growth in vitro by an action at the early stages of colony development. The parent compound isatin thiosemicarbazone and 25 other derivatives were tested, and a wide range of activity was found (50% colony inhibition from 0.07 to > 100 μg per ml of culture). High activity was not associated with any specific substituent although 1-methyl or 1-ethyl isatin groups in association with 4′4′ morpholino or 4′4′ tetramethylene thiosemicarbazone substitutions were within the most active group. No correlation between the antimarrow and known antiviral activities of these compounds was apparent. Two of the more active compounds were compared with methisazone and were shown to have the same property of selective action on the early stages of granulocyte-macrophage differentiation in culture. It is suggested that this group of compounds might possibly provide some useful antileukemia agents.
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- BAUER D. J., DUMBELL K. R., FOX-HULME P., SADLER P. W. The chemotherapy of variola major infection. Bull World Health Organ. 1962;26:727–732. [PMC free article] [PubMed] [Google Scholar]
- BAUER D. J., SADLER P. W. Derivatives of isatin beta-thiosemicarbazone with anti-viral chemotherapeutic activity against ectromelia infection. Nature. 1961 Jun 24;190:1167–1169. doi: 10.1038/1901167a0. [DOI] [PubMed] [Google Scholar]
- Bradley T. R., Metcalf D. The growth of mouse bone marrow cells in vitro. Aust J Exp Biol Med Sci. 1966 Jun;44(3):287–299. doi: 10.1038/icb.1966.28. [DOI] [PubMed] [Google Scholar]
- Bradley T. R., Sumner M. A. Stimulation of mouse bone marrow colony growth in vitro by conditioned medium. Aust J Exp Biol Med Sci. 1968 Oct;46(5):607–618. doi: 10.1038/icb.1968.167. [DOI] [PubMed] [Google Scholar]
- Levinson W., Woodson B., Jackson J. Inactivation of Rous sarcoma virus on contact with N-ethyl isatin -thiosemicarbazone. Nat New Biol. 1971 Jul 28;232(30):116–118. doi: 10.1038/newbio232116a0. [DOI] [PubMed] [Google Scholar]
- McNeill T. A. Effect of methisazone and other drugs on mouse hemophoietic colony formation in vitro. Antimicrob Agents Chemother. 1972 Jan;1(1):6–11. doi: 10.1128/aac.1.1.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McNeill T. A., Fleming W. A., McClure S. F., Killen M. Suppression of immune and hemopoietic cellular responses by methisazone. Antimicrob Agents Chemother. 1972 Jan;1(1):1–5. doi: 10.1128/aac.1.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Metcalf D. Studies on colony formation in vitro by mouse bone marrow cells. I. Continuous cluster formation and relation of clusters to colonies. J Cell Physiol. 1969 Dec;74(3):323–332. doi: 10.1002/jcp.1040740313. [DOI] [PubMed] [Google Scholar]
- Stanley E. R., Bradley T. R., Sumner M. A. Properties of the mouse embryo conditioned medium factor(s) stimulationg colony formation by mouse bone marrow cells grown in vitro. J Cell Physiol. 1971 Oct;78(2):301–317. doi: 10.1002/jcp.1040780219. [DOI] [PubMed] [Google Scholar]
- Stanley E. R., Metcalf D. Partial purification and some properties of the factor in normal and leukaemic human urine stimulating mouse bone marrow colony growth in vitro. Aust J Exp Biol Med Sci. 1969 Aug;47(4):467–483. doi: 10.1038/icb.1969.51. [DOI] [PubMed] [Google Scholar]