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. 1973 Aug;4(2):167–177. doi: 10.1128/aac.4.2.167

Reconstitution of Colicin E2-Induced Deoxyribonucleic Acid Degradation in Spheroplast Preparations

R Almendinger 1, L P Hager 1
PMCID: PMC444523  PMID: 4598217

Abstract

Spheroplasts are insensitive to colicin E2 and do not show deoxyribonucleic acid (DNA) degradation even in the presence of massive amounts of E2. However, when both endonuclease I and E2 were present, spheroplast DNA was degraded by an endonucleolytic activity which gave rise primarily to double-strand DNA cleavages, producing fragments having an average molecular weight of 9 × 106. Pancreatic ribonuclease could substitute for colicin E2 in the reconstitution system, but pancreatic deoxyribonuclease could not replace endonuclease I. However, colicin E2 could not activate transfer ribonucleic acid-inhibited endonuclease I in an in vitro system where pancreatic ribonuclease caused full stimulation.

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Selected References

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