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. 1973 Sep;4(3):320–326. doi: 10.1128/aac.4.3.320

Phleomycin-Stimulated Degradation of Deoxyribonucleic Acid in Escherichia coli

Larry Farrell a,1, Harvard Reiter a
PMCID: PMC444550  PMID: 4586146

Abstract

Phleomycin stimulates the degradation of DNA by energy-dependent endonuclease and exonuclease reactions in Escherichia coli rec+ cells and in recB− and recC− cells that lack an adenosine triphosphate-dependent nuclease functioning in the repair of ultraviolet (UV) lesions. Exonuclease activity is blocked in T4 phage-infected cells. The endonuclease reaction produces 107-dalton segments resembling those produced in colicin E2-treated cells. These differ from the random-sized segments produced in UV-irradiated cells, or the 106-dalton segments made in T4 phage-infected cells. A mutant selected for phleomycin tolerance is cross-tolerant to colicin E2, and some mutants selected for colicin E2 tolerance are cross-tolerant to phleomycin. On the basis of these cross-tolerances and the similarities between the effects of phleomycin and E2-stimulated nucleases, the suggestion is made that both agents may stimulate the same nuclease reactions in E. coli cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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