Fig. 3.

Differential modulation of neutrophil migration by super-low and low dose LPS. (A) CXCR2 expression on the Gr-1⁺ cell population isolated from the peritoneal lavages of mice 4 d post-CLP was analyzed by flow cytometry. Mice were pre-conditioned with either 5 ng/kg (SL-LPS) or 50 μg/kg (L-LPS) 1 d prior to the CLP procedure. (B) Cumulative percentages of CXCR2⁺Gr-1⁺ cells from the peritoneal lavage 4 d post-CLP. Results were expressed as the mean ± SEM, N = 4–5 mice per group as indicated in the figure. Data were representative of 3 independently performed experiments. *p < 0.05. All mice used were male and 8–10 weeks old. (C) CXCR2 expression on neutrophils in vitro was analyzed by flow cytometry after stimulation of indicated LPS concentration. Bone marrow cells were stimulated with either PBS, 100 pg/mL or 1 μg/mL LPS for 3 h. The expression levels of CXCR2 on neutrophils were quantified through flow cytometry. *p < 0.05. (D) Cumulative fold change of CXCR2⁺Gr-1⁺ cells in vitro after stimulation with LPS. Data represent 3 independently performed experiments. Results were expressed as the mean ± SEM. *p < 0.05.