Skip to main content
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1973 Nov;4(5):560–563. doi: 10.1128/aac.4.5.560

Isolation and Identification of Minor Components of Commercial Kanamycin

Paul J Claes 1, Hubert Vanderhaeghe 1, Frans Compernolle 1
PMCID: PMC444595  PMID: 4791491

Abstract

A fraction enriched in the minor components of the kanamycin complex was prepared from a commercial sample. The enriched fraction was subjected to Amberlite CG-50 and Dowex 1-X2 column chromatography. Two minor components were isolated in addition to the known A, B, and C components. These congeners were identified as paromamine and 6-o-(3-amino-3-deoxy-α-d-glucopyranosyl)- deoxystreptamine. The identification was based on degradation and mass spectral studies.

Full text

PDF
560

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. INOUYE S., OGAWA H. SEPARATION AND QUANTITATIVE DETERMINATION OF AMINO SUGAR ANTIBIOTICS AND THEIR DEGRADATION PRODUCTS BY MEANS OF AN IMPROVED METHOD OF CHROMATOGRAPHY ON RESIN. J Chromatogr. 1964 Feb;13:536–541. doi: 10.1016/s0021-9673(01)95147-7. [DOI] [PubMed] [Google Scholar]
  2. MAEDA K., MURASE M., MAWATARI H., UMEZAWA H. Structure studies on kanamycin. J Antibiot (Tokyo) 1958 Jul;11(4):163–165. [PubMed] [Google Scholar]
  3. MAEDA K., UEDA M., YAGISHITA K., KAWAJI S., KONDO S., MURASE M., TAKEUCHI T., OKAMI Y., UMEZAWA H. Studies on kanamycin. J Antibiot (Tokyo) 1957 Sep;10(5):228–231. [PubMed] [Google Scholar]
  4. Majumdar M. K., Majumdar S. K. Separation and quantitation of neomycins as free base by paper chromatography. Appl Microbiol. 1969 May;17(5):763–764. doi: 10.1128/am.17.5.763-764.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. ROTHROCK J. W., GOEGELMAN R. T., WOLF F. J. A resin chromatographic analysis for kanamycin mixtures. Antibiot Annu. 1958;6:796–803. [PubMed] [Google Scholar]
  6. Tsuji K., Robertson J. H. Gas-liquid chromatographic determination of amino-glycoside antibiotics: kanamycin and paromomycin. Anal Chem. 1970 Nov;42(13):1661–1663. doi: 10.1021/ac60295a003. [DOI] [PubMed] [Google Scholar]
  7. UMEZAWA H., UEDA M., MAEDA K., YAGISHITA K., KONDO S., OKAMI Y., UTAHARA R., OSATO Y., NITTA K., TAKEUCHI T. Production and isolation of a new antibiotic: kanamycin. J Antibiot (Tokyo) 1957 Sep;10(5):181–188. [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES