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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Gastroenterology. 2015 Feb 18;148(7):1405–1416.e3. doi: 10.1053/j.gastro.2015.02.013

Figure 5. SLC7A5-dependent mTOR localization to Salmonella-containing vacuoles and mTORC1 activity are required for enhanced Salmonella survival in CAP-D3 deficient cells.

Figure 5

A) Immunofluorescence analysis of LAMP1 (green) and mTOR (red) and bacterial nuclei (DAPI-blue) in HT-29 cells at 2 hours post-infection demonstrates increased colocalization in CAP-D3 deficient cells. B) Quantification of the percentage of mTOR+, LAMP1+, mTOR+/LAMP1+ Salmonella in HT-29 cell lines various times post-infection. C) Quantification as in (B) but in cells treated with BCH. D) The mTORC1 inhibitor, rapamycin, rescues the increased numbers of Salmonella in HT-29 cell lines at 1.5h post-infection in gentamycin protection assays.