Skip to main content
PMC home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1974 Sep;6(3):311–315. doi: 10.1128/aac.6.3.311

Antimicrobial Susceptibilities of Anaerobic Bacteria: Recent Clinical Isolates

Joseph L Staneck a, John A Washington II a
PMCID: PMC444642  PMID: 15830478

Abstract

Minimal inhibitory concentrations of clindamycin, minocycline, metronidazole, penicillin, and carbenicillin were determined by agar dilution against 150 recent clinical isolates of anaerobic bacteria. Ninety-nine percent of Bacteroides fragilis and all B. melaninogenicus, Clostridium perfringens, and Fusobacterium were inhibited by clindamycin at 3.1 μg/ml. Only 58% of other clostridial species were inhibited by this concentration of clindamycin. Minocycline at 3.1 μg/ml inhibited 72% of C. perfringens, 81% of other Clostridium species, and 66, 75, and 100% of B. fragilis, B. melaninogenicus, and Fusobacterium, respectively. Metronidazole at 12.5 μg/ml inhibited all bacteria tested. B. fragilis was resistant to both penicillin and carbenicillin at 6.2 μg/ml. Concentrations of 25 μg/ml for penicillin and 100 μg/ml for carbenicillin were needed to inhibit more than 90% of B. fragilis. Organisms other than B. fragilis were moderately or extremely susceptible to the penicillins.

Full text

PDF
311

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartlett J. G., Gorbach S. L., Finegold S. M. The bacteriology of aspiration pneumonia. Am J Med. 1974 Feb;56(2):202–207. doi: 10.1016/0002-9343(74)90598-1. [DOI] [PubMed] [Google Scholar]
  2. Bartlett J. G., Sutter V. L., Finegold S. M. Treatment of anaerobic infections with lincomycin and clindamycin. N Engl J Med. 1972 Nov 16;287(20):1006–1010. doi: 10.1056/NEJM197211162872002. [DOI] [PubMed] [Google Scholar]
  3. Bodner S. J., Koenig M. G., Goodman J. S. Bacteremic Bacteroides infections. Ann Intern Med. 1970 Oct;73(4):537–544. doi: 10.7326/0003-4819-73-4-537. [DOI] [PubMed] [Google Scholar]
  4. Bodner S. J., Koenig M. G., Treanor L. L., Goodman J. S. Antibiotic susceptibility testing of Bacteroides. Antimicrob Agents Chemother. 1972 Aug;2(2):57–60. doi: 10.1128/aac.2.2.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DUREL P., ROIRON V., SIBOULET A., BOREL L. J. Systemic treatment of human trichomoniasis with a derivative of nitro-imidazole, 8823 RP. Br J Vener Dis. 1960 Mar;36:21–26. doi: 10.1136/sti.36.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davies A. H. Metronidazole in human infections with syphilis. Br J Vener Dis. 1967 Sep;43(3):197–200. doi: 10.1136/sti.43.3.197. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Douglas R. L., Kislak J. W. Treatment of Bacteroides fragilis bacteremia with clindamycin. J Infect Dis. 1973 Oct;128(4):569–571. doi: 10.1093/infdis/128.4.569. [DOI] [PubMed] [Google Scholar]
  8. Fass R. J., Scholand J. F., Hodges G. R., Saslaw S. Clindamycin in the treatment of serious anaerobic infections. Ann Intern Med. 1973 Jun;78(6):853–859. doi: 10.7326/0003-4819-78-6-853. [DOI] [PubMed] [Google Scholar]
  9. Haldane E. V., Van Rooyen C. E. Treatment of severe bacteroides infections with parenteral clindamycin. Can Med Assoc J. 1972 Dec 23;107(12):1177–1181. [PMC free article] [PubMed] [Google Scholar]
  10. Ingham H. R., Selkon J. B., Codd A. A., Hale J. H. A study in vitro of the sensitivity to antibiotics of Bacteroides fragilis. J Clin Pathol. 1968 Jul;21(4):432–436. doi: 10.1136/jcp.21.4.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Keusch G. T., O'Connell C. J. The susceptibility of bacteroides to the penicillins and cephalothin. Am J Med Sci. 1966 Apr;251(4):428–432. doi: 10.1097/00000441-196604000-00007. [DOI] [PubMed] [Google Scholar]
  12. Levison M. E., Bran J. L., Ries K. Treatment of anaerobic bacterial infections with clindamycin-2-phosphate. Antimicrob Agents Chemother. 1974 Mar;5(3):276–280. doi: 10.1128/aac.5.3.276. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Martin W. J., Gardner M., Washington J. A., 2nd In vitro antimicrobial susceptibility of anaerobic bacteria isolated from clinical specimens. Antimicrob Agents Chemother. 1972 Feb;1(2):148–158. doi: 10.1128/aac.1.2.148. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mitchell A. A. Incidence and isolation of Bacteroides species from clinical material and their sensitivity to antibiotics. J Clin Pathol. 1973 Oct;26(10):738–741. doi: 10.1136/jcp.26.10.738. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nastro L. J., Finegold S. M. Bactericidal activity of five antimicrobial agents against Bacteroides fragilis. J Infect Dis. 1972 Jul;126(1):104–107. doi: 10.1093/infdis/126.1.104. [DOI] [PubMed] [Google Scholar]
  16. Powell S. J., Wilmot A. J., Elsdon-Dew R. Single and low dosage regimens of metronidazole in amoebic dysentery and amoebic liver abscess. Ann Trop Med Parasitol. 1969 Jun;63(2):139–142. doi: 10.1080/00034983.1969.11686609. [DOI] [PubMed] [Google Scholar]
  17. Sapico F. L., Kwok Y. Y., Sutter V. L., Finegold S. M. Standardized antimicrobial disc susceptibility testing of anaerobic bacteria: in vitro susceptibility of Clostridium perfringens to nine antibiotics. Antimicrob Agents Chemother. 1972 Oct;2(4):320–325. doi: 10.1128/aac.2.4.320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sutter V. L., Kwok Y. Y., Finegold S. M. Standardized antimicrobial disc susceptibility testing of anaerobic bacteria. I. Susceptibility of Bacteroides fragilis to tetracycline. Appl Microbiol. 1972 Feb;23(2):268–275. doi: 10.1128/am.23.2.268-275.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sutter V. L., Kwok Y., Finegold S. M. Susceptibility of Bacteroides fragilis to six antibiotics determined by standardized antimicrobial disc susceptibility testing. Antimicrob Agents Chemother. 1973 Feb;3(2):188–193. doi: 10.1128/aac.3.2.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Tally F. P., Armfield A. Y., Dowell V. R., Jr, Kwok Y. Y., Sutter V. L., Finegold S. M. Susceptibility of Clostridium ramosum to antimicrobial agents. Antimicrob Agents Chemother. 1974 Jun;5(6):589–593. doi: 10.1128/aac.5.6.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Tally F. P., Sutter V. L., Finegold S. M. Metronidazole versus anaerobes. In vitro data and initial clinical observations. Calif Med. 1972 Dec;117(6):22–26. [PMC free article] [PubMed] [Google Scholar]
  22. Thadepalli H., Gorbach S. L., Broido P. W., Norsen J., Nyhus L. Abdominal trauma, anaerobes, and antibiotics. Surg Gynecol Obstet. 1973 Aug;137(2):270–276. [PubMed] [Google Scholar]
  23. WEINSTEIN L., LERNER P. I., CHEW W. H. CLINICAL AND BACTERIOLOGIC STUDIES OF THE EFFECT OF "MASSIVE" DOSES OF PENICILLIN G ON INFECTIONS CAUSED BY GRAM-NEGATIVE BACILLI. N Engl J Med. 1964 Sep 10;271:525–533. doi: 10.1056/NEJM196409102711101. [DOI] [PubMed] [Google Scholar]
  24. Whelan J. P., Hale J. H. Bactericidal activity of metronidazole against Bacteroides fragilis. J Clin Pathol. 1973 Jun;26(6):393–395. doi: 10.1136/jcp.26.6.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wilkins T. D., Thiel T. Resistance of some species of Clostridium to clindamycin. Antimicrob Agents Chemother. 1973 Jan;3(1):136–137. doi: 10.1128/aac.3.1.136. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wilson W. R., Martin W. J., Wilkowske C. J., Washington J. A., 2nd Anaerobic bacteremia. Mayo Clin Proc. 1972 Sep;47(9):639–646. [PubMed] [Google Scholar]
  27. Zabransky R. J., Johnston J. A., Hauser K. J. Bacteriostatic and bactericidal activities of various antibiotics against Bacteroides fragilis. Antimicrob Agents Chemother. 1973 Feb;3(2):152–156. doi: 10.1128/aac.3.2.152. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES