Abstract
A number of Cephalosporium acremonium mutants blocked in the synthesis of cephalosporin C were investigated for accumulation of other β-lactam compounds. The non-cephalosporin C producers were isolated after exposing the superior cephalosporin C-producing strain M8650-4 to ultraviolet light (268 nm). One of the blocked mutants, MH63, accumulated deacetylcephalosporin C (0.4 mg/ml), deacetoxycephalosporin C (1.5 mg/ml), and penicillin N (2.7 mg/ml). In contrast, the parent of MH63 produced high levels of cephalosporin C as well as deacetylcephalosporin C (2.2 mg/ml) and penicillin N (1.0 mg/ml), but only traces of deacetoxycephalosporin C (about 0.1 mg/ml). Deacetoxycephalosporin C was isolated from the mutant strain and identified by ultraviolet light, nuclear magnetic resonance, bioactivity spectrum, and co-migration with authentic standard in three chromatography systems.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Caltrider P. G., Niss H. F. Role of methionine in cephalosporin synthesis. Appl Microbiol. 1966 Sep;14(5):746–753. doi: 10.1128/am.14.5.746-753.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dennen D. W., Carver D. D. Sulfatase regulation and antibiotic synthesis in Cephalosporium acremonium. Can J Microbiol. 1969 Feb;15(2):175–181. doi: 10.1139/m69-029. [DOI] [PubMed] [Google Scholar]
- Fujisawa Y., Shirafugi H., Kida M., Nara K., Yoneda M., Kanzaki T. New findings on cephalosporin C biosynthesis. Nat New Biol. 1973 Dec 5;246(153):154–155. doi: 10.1038/newbio246154a0. [DOI] [PubMed] [Google Scholar]
- Higgens C. E., Hamill R. L., Sands T. H., Hoehn M. M., Davis N. E. Letter: The occurrence of deacetoxycephalosporin C in fungi and streptomycetes. J Antibiot (Tokyo) 1974 Apr;27(4):298–300. doi: 10.7164/antibiotics.27.298. [DOI] [PubMed] [Google Scholar]
- Lemke P. A., Nash C. H. Mutations that affect antibiotic synthesis by Cephalosporium acremonium. Can J Microbiol. 1972 Feb;18(2):255–259. doi: 10.1139/m72-038. [DOI] [PubMed] [Google Scholar]
- RUSSO-ALESI F. M., SHERMAN C., KELLY J. M., FERRARI A. The simultaneous determination of sample and blank by means of dual and differential colorimetry in the continuous analysis of penicillin in fermentation media. Ann N Y Acad Sci. 1960 Jul 22;87:822–829. doi: 10.1111/j.1749-6632.1960.tb23240.x. [DOI] [PubMed] [Google Scholar]