Skip to main content
NCBI home page
Antimicrobial Agents and Chemotherapy logoLink to Antimicrobial Agents and Chemotherapy
. 1974 Oct;6(4):471–473. doi: 10.1128/aac.6.4.471

Comparison of the In Vitro Activity of BL-P1654 with Gentamicin and Carbenicillin Against Pseudomonas aeruginosa

Chatrchai Watanakunakorn 1, Thomas Bannister 1
PMCID: PMC444673  PMID: 4157347

Abstract

The in vitro activity of 6-[d-α-(3 guanylureido)-phenylacetamido]-penicillanic acid (BL-P1654) was evaluated against 117 clinical isolates of Pseudomonas aeruginosa, many of which were known to be resistant to both gentamicin and carbenicillin. BL-P1654 was two to eight times more active than carbenicillin against P. aeruginosa. However, all 28 highly carbenicillin-resistant isolates (minimal inhibitory concentration [MIC] > 500 μg/ml) were also highly resistant to BL-P1654. When an MIC of 32 μg/ml or less and a zone of inhibition of 12 mm or more by a 10-μg disk were used as criteria indicating susceptibility to BL-P1654, the false-resistance rate by the disk test was 10.6% and the false-susceptibility rate was 4.2%. The combination of BL-P1654 and gentamicin was synergistic against 45 of 70 isolates of P. aeruginosa tested, but synergism was demonstrated against only 4 of 24 highly gentamicin-resistant (MIC > 63 μg/ml), 1 of 12 highly BL-P1654-resistant (MIC > 250 μg/ml) isolates, and none of nine isolates highly resistant to both of these antibiotics.

Full text

PDF
471

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  2. Bodey G. P., Horikoshi N., Rodriguez V. Human pharmacology of 6-[D-alpha-(3-guanylureido)-phenylacetamido]-penicillanic acid (BL-P1654). Antimicrob Agents Chemother. 1974 Apr;5(4):366–370. doi: 10.1128/aac.5.4.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bodey G. P., Stewart D. In vitro studies of semisynthetic alpha-(substituted-ureido) penicillins. Appl Microbiol. 1971 Apr;21(4):710–717. doi: 10.1128/am.21.4.710-717.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ericsson H. M., Sherris J. C. Antibiotic sensitivity testing. Report of an international collaborative study. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;217(Suppl):1+–1+. [PubMed] [Google Scholar]
  5. Greene W. H., Moody M., Schimpff S., Young V. M., Wiernik P. H. Pseudomonas aeruginosa resistant to carbenicillin and gentamicin. Epidemiologic and clinical aspects in a cancer center. Ann Intern Med. 1973 Nov;79(5):684–689. doi: 10.7326/0003-4819-79-5-684. [DOI] [PubMed] [Google Scholar]
  6. Knothe H., Krcméry V., Sietzen W., Borst J. Transfer of gentamicin resistance from Pseudomonas aeruginosa strains highly resistant to gentamicin and carbenicillin. Chemotherapy. 1973;18(4):229–234. doi: 10.1159/000221266. [DOI] [PubMed] [Google Scholar]
  7. Lowbury E. J., Lilly H. A., Kidson A., Ayliffe G. A., Jones R. J. Sensitivity of Pseudomonas aeruginosa to antibiotics: emergence of strains highly resistant to carbenicillin. Lancet. 1969 Aug 30;2(7618):448–452. doi: 10.1016/s0140-6736(69)90163-9. [DOI] [PubMed] [Google Scholar]
  8. Matsen J. M., Lund M. E., Brooker D. C. Comparison and evaluation of carbenicillin disks in diffusion susceptibility testing. Antimicrob Agents Chemother. 1974 Jun;5(6):599–606. doi: 10.1128/aac.5.6.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Phair J. P., Tan J. S., Watanakunakorn C., Schwab L., Sanders L. W. Carbenicillin treatment of Pseudomonas pulmonary infection. Use in children with cystic fibrosis. Am J Dis Child. 1970 Jul;120(1):22–25. doi: 10.1001/archpedi.1970.02100060056006. [DOI] [PubMed] [Google Scholar]
  10. Phair J. P., Watanakunakorn C., Bannister T. In vitro susceptibility of Pseudomonas aeruginosa to carbenicillin and the combination of carbenicillin and gentamicin. Appl Microbiol. 1969 Sep;18(3):303–306. doi: 10.1128/am.18.3.303-306.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Traub W. H., Raymond E. A. Susceptibility of Pseudomonas aeruginosa to carbenicillin. Appl Microbiol. 1970 Oct;20(4):630–632. doi: 10.1128/am.20.4.630-632.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Washington J. A., 2nd, Hall M. M., Fausch C. J., Brackin K. L. In vitro activity of carbenicillin against Pseudomonas using the disc diffusion method. Mayo Clin Proc. 1973 Oct;48(10):718–721. [PubMed] [Google Scholar]

Articles from Antimicrobial Agents and Chemotherapy are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES