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. 1974 Oct;6(4):474–478. doi: 10.1128/aac.6.4.474

Binding of [14C]Erythromycin to Escherichia coli Ribosomes

Sidney Pestka 1
PMCID: PMC444674  PMID: 4157348

Abstract

Erythromycin binding to Escherichia coli ribosomes required K+ and Mg2+. Under optimal conditions, the dissociation constant for erythromycin binding to E. coli ribosomes was found to be 1.0 × 10−8 M and 1.4 × 10−8 M at 24 C and 5 C, respectively. One molecule of [14C]erythromycin was bound to each 70S ribosome at equilibrium. Binding of erythromycin to ribosomes was rapid and reversible. The specific rate constants for the forward and reverse reactions were 1.7 × 107 liters per mol per min and 0.15 per min, respectively.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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