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. 1974 Dec;6(6):680–684. doi: 10.1128/aac.6.6.680

3-N Enzymatic Acetylation of Gentamicin, Tobramycin, and Kanamycin by Escherichia coli Carrying an R Factor

François Le Goffic 1,2, Annie Martel 1,2, Janine Witchitz 1,2
PMCID: PMC444719  PMID: 4615629

Abstract

A new R factor-mediated gentamicin acetyltransferase obtained from a clinical isolate of gentamicin-, tobramycin-, and kanamycin-resistant Klebsiella was characterized. R factor R 176 was transferred to Escherichia coli K-12 LA 290 from a Klebsiella. Resistance transfer, minimal inhibitory concentrations of various aminoglycoside antibiotics, enzymatic inactivation, and the isoelectric point of the enzyme obtained were determined. We found that R factor R 176 could govern the enzymatic 3-N acetylation of gentamicin, tobramycin, and kanamycin, and isoelectric point determinations suggested that the enzymes governed by R 176 and R 135 are different. The genes responsible for resistance to gentamicin, tobramycin, and kanamycin govern the synthesis of an isoenzyme of AAC(3)I, which we propose to call AAC(3)II.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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