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. 1974 Dec;6(6):770–776. doi: 10.1128/aac.6.6.770

Characterization of the Binding of Amphotericin B to Saccharomyces cerevisiae and Relationship to the Antifungal Effects

Janina Kotler-Brajtburg 1, Gerald Medoff 1, David Schlessinger 1, George S Kobayashi 1
PMCID: PMC444734  PMID: 4615631

Abstract

Based on the enhanced fluorescence of amphotericin B in acid solutions, a quantitative assay for this polyene antibiotic has been developed that is sensitive and linear in the range of 0.1 to 10.0 μM. The binding of amphotericin B to Saccharomyces cerevisiae was assayed under various conditions as the amount bound to cells in a dialysis chamber or after centrifugation. Two types of binding were defined: weak, reversible binding occurred at 0 C or higher temperatures and even in the presence of inhibitors of energy metabolism, whereas strong, irreversible binding did not occur at 0 C and was inhibited when energy metabolism was blocked. Only strong binding was correlated with cell killing. Weak binding probably involves the outer layer of the membrane; strong binding probably requires disruption of hydrophobic regions of the cell membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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