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. 1974 Dec;6(6):825–830. doi: 10.1128/aac.6.6.825

Evidence for Participation of Autolysins in Bactericidal Action of Oxacillin on Staphylococcus aureus

Gary K Best 1, Norma H Best 1, Ann V Koval 1
PMCID: PMC444744  PMID: 4498775

Abstract

A comparison of the autolytic enzyme activity in Staphylococcus aureus strains that differ markedly in their rates of lysis and killing after exposure to oxacillin has been made. Log-phase cells of the clinical isolate that is tolerant to oxacillin inhibition were found to contain a level of autolytic enzyme activity comparable to that in a sensitive strain. This autolysin from log-phase cells was recovered after a single freeze-thaw cycle and assayed by using both native and penicillin (un-cross-linked) mureins. These same assays, however, revealed a significant difference in autolysin activity extractable from the two strains if the cells were inhibited by oxacillin. Under these conditions, the S. aureus strain that is susceptible to the killing and lytic effects of oxacillin had considerably more activity on penicillin murein than did the tolerant organism. These results provide evidence that hydrolytic enzymes on the cell surface are required to augment the wall damage initiated by oxacillin and other β-lactam antibotics to produce a bactericidal effect.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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