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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Apoptosis. 2015 Jul;20(7):921–929. doi: 10.1007/s10495-015-1129-x

Fig. 3. The role of TNF-α in cisplatin induced injury in NT3 and C2 cells.

Fig. 3

(A) Real time PCR of TNF-α in NT3 and C2 cells. The result is normalized to NT3 cells (N=3). Immunoblot analysis (B) and quantification of TNF-α (C) in whole cell lysates obtained from cells treated with 150 μM cisplatin for indicated time periods (N=2). The β-actin blot serves as the loading control. (D) Cell viability determined by MTT assay in cells treated with indicated concentrations of pentoxifylline and 150 μM cisplatin for 24 h (N=3). (E) Cell viability determined by MTT assay in cells treated with 150 μM cisplatin alone or in combine with 200 μg/ml pentoxifylline for the indicated time. The results are presented as the viability percentage of untreated control in SF media. The asterisks indicate significant differences between NT3 and C2 cells.