TABLE 1.
Characterization of particle motility in axons and virus yield in cell bodies during high- and low-MOI retrograde PRV 180 infection
| MOI | No. of capsids/1.65 × 10−2 mm2 | Motility (% total particles)a,b | Virus yield (PFU/ml)a,c |
|
|---|---|---|---|---|
| 24 hpi | 48 hpi | |||
| 100 | 325 ± 51.8 | 44.5 ± 3.8 | 8.3 × 104 ± 3.4 × 104 | 1.1 × 106 ± 2.1 × 105 |
| 10 | 43.3 ± 16.8 | 46.5 ± 3.8 | 3.6 × 104 ± 1.2 × 104 | 5.5 × 105 ± 1.6 × 105 |
| 1 | 2.4 ± 2.5 | ND | <5 | 3.5 × 104 ± 6.7 × 104 |
| ≤0.1 | 0–1 | ND | <5 | <5 |
a
Values are means ± STDEV.
b
ND, not detected.
c
The virus yield detection limit is 5 PFU/ml.