Figure 2. Western blots probing for the presence of a nucleoporin (Nup62), three nuclear antigens (lamin LIII, nucleoplasmin, and the 70-kDa subunit of RPA), and the ER integral membrane protein, ribophorin.
Fractions 1–4 correspond to those in Figure 1C and contain AL or AL fractions derived from the equivalent of 0.25 µl of extract, while lane C contains 0.025 µl of cytosol as a marker.