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Supporting Information
Immunohistochemical staining of the coronal sections of rat and mouse brains, containing the median preoptic nucleus (MnPO) (A) and median eminence (B) with anti-rat Nax [12] and anti-mNax antibodies. Immunohistochemical staining was performed as described in S5 File. Neither rat nor mouse MnPO was negative for Nax (A). On the other hand, the median eminence was clearly stained with both antibodies (B). AC, anterior commissure. Scale bars, 200 μm.
(TIF)
Reference
- 1. Matsumoto M, Hiyama TY, Kuboyama K, Suzuki R, Fujikawa A, Noda M (2015) Channel Properties of Nax Expressed in Neurons. PLoS ONE 10(5): e0126109 doi:10.1371/journal.pone.0126109 [DOI] [PMC free article] [PubMed] [Google Scholar]
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Supplementary Materials
Immunohistochemical staining of the coronal sections of rat and mouse brains, containing the median preoptic nucleus (MnPO) (A) and median eminence (B) with anti-rat Nax [12] and anti-mNax antibodies. Immunohistochemical staining was performed as described in S5 File. Neither rat nor mouse MnPO was negative for Nax (A). On the other hand, the median eminence was clearly stained with both antibodies (B). AC, anterior commissure. Scale bars, 200 μm.
(TIF)