Figure 6. Tip cell defects in Wnt/β-catenin-deficient PHBCs.

(A) Scatterplot of a microarray comparison of wild-type and gpr124^s984/s984 embryos at 48 hpf. Each dot refers to the relative signal intensity of a given probe in mutant vs wild-type embryos. Circles identify independent probes for vegfaa, vegfab, and vegfc. (B) Endothelial cell number in the PHBCs of wild-type and gpr124^s984/s984 embryos at 30 hpf. kdrl:NLS-GFP⁺ nuclei were counted on one side of the embryos. (C) Dorsal views of Tg(pdgfrb:mCitrine);Tg(kdrl:ras-mCherry) wild-type and gpr124^s984/s984 embryos at 40 hpf. (D) Stills from Video 3 recording sprouting angiogenesis in the hindbrain of Tg(kdrl:GFP) wild-type and gpr124^s984/s984 embryos. Below are kymograph plots showing a time course of pixel intensity across the yellow virtual lines running 15 µm dorsal to the PHBC (from the anterior MCeV to the posterior PCeV [posterior cerebral vein]) and depicted in the upper left panel. (E) Lateral views of Tg(kdrl:lifeact-GFP);Tg(kdrl:NLS-mCherry) wild-type and gpr124^s984/s984 hindbrains at 30 hpf. Arrows point to actin-dense structures forming in the PHBC vessel wall. Scale bars, 50 µm. Values represent means ± SD.

DOI: http://dx.doi.org/10.7554/eLife.06489.014

Figure 6—figure supplement 1. Endothelial cell number in wild-type and gpr124^s984/s984 brain vessels at 54 hpf.

Maximal intensity projection of a confocal z-stack of the Tg(kdrl:NLS-GFP) wild-type and gpr124^s984/s984 cranial vasculature at 54 hpf (left panels). kdrl:NLS-GFP⁺ EC nuclei were counted in the PHBCs, ventral PHBC-BA connections, BA and CtAs (right panels). In all panels, values represent means ± SD (*p < 0.05; **p < 0.01; Mann–Whitney test) of a minimal of five embryos.

DOI: http://dx.doi.org/10.7554/eLife.06489.015