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. 2015 May;6(Suppl 1):S26–S32.

Update Ayurveda 2014 Abstracts: Poster Presentation

PMCID: PMC4456680
J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/01: Antimalarial activity of Caesalpinia crista

Chitralekha L. Mane, S. S. Sant

Sharir Kriya Dept., Government Ayurved College, Nanded, India

Email: dr.chitramane101088@gmail.com

Malaria is one of the most life-threatening infectious diseases worldwide and claims the millions of people's life each year. The appearance of drug-resistance Plasmodium falciparum has made the treatment of malaria increasingly problematic, and thus, it is a dire need to search the new alternatives of current drugs. Latakaranja i.e. Caesalpinia Crista from family Caesalpiniaceae is one of the herbs mentioned in all Ayurvedic Texts and traditionally used for periodic fever. The CH2Cl2 extract of the seed kernels of Caesalpinia crista, which exhibited promising antimalarial activity against Plasmodium. 44 cassane- and norcassane-type diterpenes isolated from Caesalpinia crista evaluated for their antimalarial activity against the malaria parasite Plasmodium falciparum. Most of the tested diterpenes displayed antimalarial activity and norcaesalpinin E showed the most potent activity. The combination of its roasted seed powder and Pippali 1:1 proportion given with Honey approximately 0.5 gm thrice a day for three days duration. Another combination recommended for malaria is the powders of marich and latakaranj. The splenic enlargement due to malaria responds well to Latakaranj.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/02: Role of Panchakarma in surgical diseases: A comprehensive approach

Amit Kumar Negi, P.Hemantha Kumar, B. Swapna

National Institute of Ayurveda, Jaipur, India

Email: amitnegiayurveda@gmail.com

Background: On par with surgical techniques used to uproot the pathology of a disease, Panchakarma, a major therapeutic contribution of Ayurveda, concedes ultimate bio-purificatory methods with radical action in the management of a disease. In view of their importance, Acharya Sushruta incorporated Panchakarma procedures in the management of surgical diseases. Objective: To explore the Panchakarma procedures those can be practiced safely in surgical diseases. Materials and Methods: Panchakarma is indicated in conditions like Gudabhramsha, Yonibhramsha, Arshas, Bhagandar, Parikartika, Vidradhi, Bhagna, among Shashti upakramas of vrana etc. Vamanakarma in Dushtavrana, Virechanakarma in bhagandara, osteomyelitis; swedana karma in soft tissue injury and post plaster removal after fracture, Basti (Enema) in patient preparation for shalya karma, Anuvasanavasti in parikartika, Uttaravasti in vatashteela, Kativasti and greevavasti in lumbar spondylosis, cervical spondylosis respectively, Vranavasti in chronic non healing ulcers and osteomyelitis, are some of the conditions where panchakarma is indicated. Raktamokshana, performed in amajavranashopha, thrombosed external haemorrhoids is considered by Sushruta as one of the panchakarma procedures. Results: Panchakarma therapy performed either as main line or as an adjuvant in any surgical disease eliminates the causative factors completely or alleviate to the extent that the disease may not need further surgical intervention, which is conducive enough to the diseased. Conclusion: In many surgical diseases, Panchakarma procedures being performed at pre-operative, operative and post-operative levels play important role either as the first line of treatment or as an adjunct to the main treatment.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/03: Development and validation of assessment criteria for Agni – inferences from a pilot study

Ashwathykutty. V. Pawankumar Godatwar, Reetu Sharma

Department of Rog Nidan and Vikriti Vijnan, National Institute of Ayurveda, Jaipur, India

Email: toashwathy@gmail.com

Back ground: The tools which were developed till date to assess Agni were identifying the Agnibala, not the state of Agni. These conventional methods which assess the degree of Agnibala do not offer any help in identifying the doshas involved in the derangement of Agni. Considering the lacunae in the current methods, a short questionnaire was developed to assess the four states of Agni. Objectives: To develop the questionnaire and validate it in different steps. Materials and Methods: For framing the questionnaire, the variables were identified and proper scoring was done. Validation was done in different phases. Survey study was conducted in 45 healthy volunteers, aged between 20-40 yrs, of either sex, randomly selected from the NIA campus, Jaipur. The internal consistencies among the questions were assessed using the crohnbach's α using SPSS. Results: Totally 45 volunteers were analysed. 26 individuals were found to have samagni, 13 were with mandagni, 4 were with vishamagni and 2 were of Tishnagni. In statistical analysis, it is found that the Crohnbach'sα is. 639 which shows an acceptable limit of internal consistency. Conclusion: Agni being the key factor in ayurvedic treatment, it should be thoroughly understood and assessed before the treatment is decided. This can help us identifying the most suitable medicine in its proper dose and dosage form. The study can throw some light in this regard and can be performed with further clarifications and advancements.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/04: Junk food habit as a risk factor in Grahani dosha: An epidemiological study

Atul Jain, Nadira Khatoon, Sevatkar Balkrishan, Sisir Kumar Mandal, Pawankumar Godatwar

Department of Roga nidana and Vikriti vijnana, National Institute of Ayurveda, Jaipur, India

Email: atuljainu@gmail.com

Background: In the recent years, there has been an unprecedented increase of incidences related to gastrointestinal system due to changing in life style, diet pattern, behavioural pattern and mental stress and strain. One such type of common gastrointestinal disorder is Grahani Dosha that is a psychosomatic disorder caused by regular intake of junk food that weakens the digestive fire and disharmony is produced between the digestion and absorption of body. Objective: To conduct a Survey study of junk food as a risk factor in Grahani patients. Materials and Methods: A survey study of total 250 Volunteers was conducted at NIA OPD, IPD, various camps in Jaipur, Rajasthan and certain junk food outlets. Volunteers were screened on the basis of prepared questionnaires. Results: On survey study, various risk factors that are considered significant for the selection of Grahani patients like Junk food user including addicted, Frequent and Regular 60%, Problem after taking junk food 60%, Heart burn 38.8%, Delayed digestion of food 54%, Fatigued/Lethargic 45.2%, Gurgling sound in abdomen 52.8%, Tendency of flatulence 34.4%, Altered bowel habit 29.2%, Disrupted agni 44%, altered Jarana sakti 39.2% and altered Abhyavarana sakti 46% subjects. Conclusion: Maximum prevalence was found in patients leading sedentary life styles and emotionally disturbed patients were more prone for the disease. It was concluded that continuous intake of junk food is risk factor for Grahani roga rather than sometimes intake of junk food.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/05: Concept of Herbal Nebulizer in Ayurveda

Gaurav Soni, J. Manohar, Sandeep Lahange

Department of Sharir Rachana, National Institute of Ayurveda, Jaipur, India

Email: gauravsonilko@gmail.com

Background: Ayurveda by itself can tackle the needs of health care today, though some adaptation can be more valuable in today's world. Ayurveda has a 3 tier structure i.e. Tatwa (Principle), Shastra (Theory) and Vyavahara (Practice). Vyavahara is the development of new applications according to particular problems that manifest differently. Medicinal requirement is not always fulfilled by systemic route so there is need to change the route to administer the drug. Objective: In Ayurveda proper Nebulization therapy has been not explained but the process of Dhumpana and Nasya may be analogous in some extent. Here in this work we had discussed various facts and factors for establishing new route of drug administration in form of herbal nebulizer. Materials and Methods: Literal review of Ayurvedic Classics and other relevant contemporary science literature along with Internet material and research papers. Results: Inhalation as a route for the administration of drug especially for the management of respiratory disorders is as old as Ayurveda. Technique of nebulizer in form of aerosol through Nebulization apparatus for herbal drugs is better route for respiratory distress. Conclusion: This work only gives the glimpse of concept of inhalation therapy described in Ayurvedic classics and establishment of newer concept of drug administration route i.e. through Nebulization. Yet a herculean work is needed for better and precise way of converting herbal drugs in form suitable for inhalation therapy.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/06: Role of Yastimadhu Ghruta Tarpan in Computer Vision Syndrome (CVS)

Kavita Thorat

Dr. DY Patil College of Ayurved and Research Centre, Nerul, Navi Mumbai, India

Email: drkavitathorat@gmail.com

Background: Eyes are the doors of wisdom; immense importance has been given to the eyes in our classics. In this era of computers, radiations these doors are affected first and fast. So how are we going to face the challenge of preserving vision? The answer is in the hands of ayurveda, one of the oldest organized ways of life rather than just a system of medicine, the vision of ayurvedic fraternity is to restore vision to entire world. After all its ayurveda which proclaimed to the entire world about the preventive aspect for the first time “Swasthasya Swasthya Rakshanam”. Objectives: To assess the effect of Yastimadhu Ghruta Tarpan in Computer Vision Syndrome (CVS). Materials and Methods: Drugs – Yastimadhu Ghruta, Udid flour. Tarpan procedure was performed according to samhitas. 30 patients were studied. All necessary examinations were carried out before and after the treatment. All the findings are recorded on standard proforma specially prepared for this purpose. Result: Yashtimadhu ghruta tarpan significantly reduce symptoms of CVS Conclusion: Yashtimadhu ghruta tarpan reduce symptoms of CVS for longer duration.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/07: Hepatoprotective potential of lycopene in paracetamol induced hepatotoxicity: An experimental study

Manjunatha T A, Joshi SS

Department of Pharmacology and Therapeutics, Seth G S Medical College and K E M Hospital, Mumbai, India

Email: drmanju186@yahoo.co.in

Background: Lycopene is a carotenoid present in many fruits and vegetables, predominantly tomato (Solanum lycopersicum), with proven antioxidant properties. Objective: To evaluate the hepatoprotective activity of lycopene in paracetamol induced hepatotoxicity in Wistar rats. Materials and Methods: Lycopene (10% powder) in doses of 5 mg/kg/day and 10 mg/ kg/day, 0.5% carboxymethylcellulose (vehicle control) and silymarin 50 mg/kg/day (positive control) were administered orally for 7 days in Wistar rats, followed by administration of single oral dose of paracetamol 2 g/kg. Liver function parameters and morphological features of liver were measured, and histopathological examination of liver was carried out. Parametric data in different treatment groups was statistically analyzed using one way ANOVA (analysis of variance) with post-hoc Tukey's test (P < 0.05). Non parametric data was analyzed using Kruskal-Wallis test (P < 0.05). Results: There was significant increase in serum levels of bilirubin, and transaminases with decrease in total protein level, in the disease control, vehicle control and lycopene 5 mg/kg/day groups, reflecting liver injury with paracetamol. A significant decrease in serum levels of the markers and significant increase in total protein was observed in lycopene 10 mg/kg/day and Silymarin treated groups, indicating recovery of hepatic cells. Histopathological examination of disease control, vehicle control, and lycopene 5 mg/kg/ day groups, showed variable degrees of fatty degeneration and cellular necrosis whereas lycopene 10 mg/kg/day and silymarin treated animals revealed normal hepatic architecture without any cellular necrosis and fatty infiltration. Conclusion: Lycopene 10 mg/kg/day afforded significant protection against paracetamol induced hepatotoxicity in Wistar rats.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/08: Antibacterial activity of hydro alcoholic extract of Gymnema sylvestre

Rajesh Kumar Suman, Manjusha Borde, Ipseeta Ray Mohanty, Vijay Mane, Y A Deshmukh, A D Urekar

MGM Medical College, Kamothe, Navi Mumbai, lindia

Email: manjusha_dhande@yahoo.co.in

Background: Gymnema sylvestre (Family- Asclepiadaceae) is one of the important medicinal plants of India widely used in the Indian System of Medicine. The leaves of Gymnema sylvestre possess anti-inflammatory, anti- helmenthic, laxative, cardio-tonic, expectorant and antipyretic properties. Objective: To evaluate the antibacterial activity of hydro alcoholic leaves extract of Gymnema Sylvestre against E. coli, and Acinatobactor. Material and Methods: The hydroalcoholic extract of leaves of Gymnema Sylvestre was purchased from Sanat Pharmaceutical, New Delhi. The extract was tested against standard strain and clinical isolate of E. coli, and Acinatobactor at different concentration (1 mg, 2 mg, 4 mg and 8 mg) by Agar well diffusion technique using Muller Hinton agar. The routine antibiotic Cefotaxime was used as control drug against test bacteria under similar condition. Results: The hydro alcoholic leaves extract of Gymnema Sylvestre at concentration of 1mg, 2 mg, 4 mg and 8 mg were tested against E. coli and acinatobactor. Gymnema Sylvestre demonstrated significant zone of inhibition at different concentration (1mg, 2 mg, 4mg and 8 mg) against E. coli (ATCC 25922) and E. Coli from clinical isolate (urine) as compared with control drug. The herbal extract also showed significant antimicrobial activity against Acinatobactor from clinical isolate (Tracheal Secretion) as compared with control drug under similar condition. Conclusion: The herb Gymnema Sylvestre has a significant antibacterial property against E. coli and Acinatobactor.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/09: Indian festivals with the view of Ayurveda

Priti Patil, Baldev Kumar Dhiman

Department of Basic Principles, National Institute of Ayurveda, Jaipur, India

Email: preets297@gmail.com

Background: The Sanskrit word for festivals is utsava means removal of worldly sorrows. The condition of human body depends on the continuous interaction between internal and external factor. Based on this principle, festival delicacies and of course, the diet and lifestyle regimen (dinacharya and ritucharya) of Ayurveda have been adapted to seasonal changes. Our ancients were well informed about science behind festivals and also about human tendencies so they just tried to link up festivals with religious values. Objectives: (1) To study the actual logic behind celebration of Indian festivals. (2) To study the linkage of some festival with seasonal changes. (3) To explore the scientific basis of festivals apart from religion aspect. Materials and Methods: All the matters of some commonest festivals are collected from the books available and from local traditions followed in home. Ayurvedic dincharya and ritucharya matter is collected from Ayurvedic samhitas and correlation of gathered matter was done to discover the purpose of how traditions meet technology. Result and Conclusion: Various scientific facts came to knowledge when the study done and scientific basis behind celebration of festival are explored out. Our ancients celebrating festivals for avoiding the seasonal diseases creating havoc in day today's life. And as they linked festival with religious values, it becomes easy to continue it for long time i.e. even up to this generation and definitely this tradition will be continued for years after year.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/10: Bioactive fraction of Couroupita guianensis prevent AGE formation and protein oxidation against glucose-induced protein glycation

Shweta Lotankar, Gauresh Somani, Sadhana Sathaye

Pharmacology Research Laboratory-II, Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, India

Email: sslotankar13@gmail.com

Background: Advanced glycation end products are glycated proteins responsible for the development and worsening of diabetic complications. Objective: The present experiment has measured the effect of Couroupita guianensis (CG) extract and its fraction during the early stage, middle stage and last stage of AGE formation. Material and Methods: The methanol extract of Couroupita guianensis (MCG) was prepared by soxhlet extraction. It was further extracted with ethyl acetate (EA), butanol and water to afford three fractions. Glycation inhibitory activity of CG fraction was determined by using an in vitro glucose-bovine serum albumin (BSA) assay. The protein oxidation was examined using the level of protein carbonyl content and thiol group. Identification of the bioactive components was attempted using different analytical techniques such as HPTLC, HPLC and LC-MS. Results: When BSA was incubated with glucose, the significant increase in fluorescence intensity was observed after 21 days of the experiment. When MCG and its fraction at different concentrations were added to reaction mixture containing BSA/Glucose, the fluorescence intensity was significantly decreased in a concentration-dependent manner. Amongst all the fractions investigated, EACG showed decrease in the fluorescence intensity at less concentration followed by butanol fraction. Further, CG fraction also prevented oxidative protein damages, including effects on protein carbonyl formation and thiol oxidation of BSA. Conclusion: EACG could be used as a preventive measure against glycation-associated complications in diabetes.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/11: Novel and efficient method for extraction and isolation of parthenin from Parthenium hysterophorus

Siddeshwar H Pawar, S. R. Kulkarni

Department of Pharmacognosy and Phytochemistry, Bombay College of Pharmacy, Kalina, Santacruz (E), Mumbai, India

Email: 2008siddeshwarp@gmail.com

Background: Plant Parthenium hysterophorus contains parthenin which is responsible for various pharmacological activities such as anticancer, antibacterial, insecticidal etc. Objective: To make parthenin standard quickly and easily; there was a need of novel method of extraction of parthenin from Parthenium hysterophorus for study of its pharmacological activities on large scale. Materials and Methods: So current study was focused on efficient method of extraction and isolation of parthenin and other allied components from Parthenium hysterophorus. Various methods have been tried for isolation of parthenin from Parthenium hysterophorus extract includes; direct column chromatography of plant extract, solvent partitioning method, treatment of extract followed by column chromatography and by non solvent addition method. This isolated compound was characterized using M.P., HPTLC, HPLC, UV, IR, NMR and GC-MS. Results: Isolated compound was confirmed as parthenin. Non solvent addition method gave good result on the basis of time, yield and other expenditure as compared to other methods. Conclusion: The present study concludes that Non solvent addition method can be considered as novel method for extraction of parthenin from Parthenium hysterophorus

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/12: A Pilot study, the effect of classical Virechan karma in the management of Hypertension. Case series

Ujwala Hivale, Santoshkumar Bhatted

Department of Panchakarma, National Institute of Ayurveda, Jaipur, India

Email: ujwala.hivale27@gmail.com

Background: Hypertension one of the grave conditions causes 6% of death worldwide. About 800 million people in the world are suffering from hypertension. Various studies reveal that there is a strong relation between hypertension and coronary artery disease. Though number of antihypertensive drugs are available but the complication of hypertension are frequently observed which life are threatening. Objective: The present study was done to assess the effect of complete classical virechana karma in hypertension. Materials and Methods: Three patients suffering with hypertension were selected randomly and classical virechana was given. Results: Moderate reduction in both systolic and diastolic blood pressure was noted after the treatment. Conclusion: Classical Virechana treatment is moderately effective in reducing blood pressure.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/13: A conceptual study to explore the scientific basis of Bhutavidya in the context of Brihattrayi

Deepak Rahangdale, Yogendra Kamble, Baldev Kumar

Department of Basic Principle, National Institute of Ayurveda, Jaipur, India

Email: deepak23aug87@gmail.com

Ayurveda is the science of life and among the eight paths, of whose Bhutavidya deals with the mainly imbalance of mind and its diseases and treatment. To evaluate the literature related to bhutavidya from brihattrayi and also to establish the underline cause effect theory of treatment modalities of bhutavidya with logical reasoning. Collection and collation of literature of bhuta-vidya from brihattrayi. Its development and cause with the purview of sociology Incorporation of bhutavidya in brihattrayi. Cause effect relationship accordance with pathology and treatment establishing current relevancy of the topic and the treatment. Bhutavidya deals with all sorts of problems that have no direct physical explanation in terms of dosha, dhatu or mala and also concerned with diseases of unknown cause which are designated as idiopathic. Bhutavidya is not much different from Psychotherapy and Hypnotism of the modern and along with mind power. Bhutavidya is somewhat manasa vikara explained in Ayurveda but keen observation and thinking leads to conclude that bhutavidya is described under all the categories i.e. nija, agantuja and manasa. As the dinacarya, ritucarya, sadvritta, along with pathya-apathya and vyayama explained in Ayurveda will help to achieve physical and mental health.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/14: Multilevel qualitative LC − MS metabolomic approach to study the responses of Tinospora cordifolia treatment in hyperlipidemic patients

Amey Shirolkar1, A Pagase1, C Mulye1, R Dabur2

1Department of Biochemistry, National Research Institute of Basic Ayurvedic Sciences (NRIBAS-CCRAS), Kothrud, Pune, Maharashtra, 2Department of Biochemistry, Maharshi Dayanand University (MDU), Rohtak, Haryana, India

Email: amey_593@rediffmail.com

Background: Tinospora cordifolia (willd.) miers juice (TCJ) possesses antihyperlipidemic properties and its mechanism need to be studied. Objective: A multicompartmental nontargeted LC − MS metabolomics approach was used to study the plasma metabolic responses of hyperlipidemic patients after treatment with TCJ. Materials and Methods: 20 individuals (8 hyperlipidemic) underwent TCJ treatment (100ml decoction/individual/day). Plasma and plasma extracted lipid samples obtained from healthy, hyperlipidemic (HPL) and TCJ treated hyperlipidemic (HPL-PT) individuals were processed and analyzed in a standardized UPLC-QTOFMS instrument. A supervised multivariate data analyses with PCA and PLS-DA statistical models were performed for pattern recognition. The method was validated by analyzing several known, unknown, abundant and minute metabolites from experimental categories. Metabolic pathways were drawn to understand the molecular mechanism of TCJ action. Results: PCA and PLS-DA revealed marked effects of the drug on plasma metabolites. In plasma positive category carnitine, N-methyltryptamine, 7a-hydroxy-3-oxo-5b-cholanoic acid, 7-Hydroxy-3-oxocholanoic acid, cholic acid glucuronide, lactosylceramide were downregulated in HPL-PT; sphinganine, L-acetylcarnitine, L-phenylalanine and proline betaine were upregulated. In plasma negative category several fatty acid derivatives were downregulated; guanidoacetic acid and nicotinamide ribotide were upregulated. In plasma lipid positive group alhpa-tocopheronolactone was downregulated; in plasma lipid negative group propionyl-CoA, 3-hydroxybutyryl-CoA and angiotensin-ll were down regulated (FC > 2.0). Conclusions: The LCESI-QTOFMS/MS method in positive and negative ion polarity mode helps to qualitatively study the plasma metabolites post TCJ treatment in HPL without sample derivatization. The effect of TCJ was observed across several metabolic pathways. Several unknown metabolites obtained in this study would assist in finding newer plasma biomarkers of hyperlipidemia.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/15: A comparative clinical study of the Varnya effect of Kumkumadi Taila and its cream preparations’

Kavita Kumari Jain, Monika Sharma, Rajendra Prasad Sharma

Department of Rasashastra and Bhaishajya Kalpana, National Institute of Ayurveda, Jaipur, India

Email: 123123kavijain@gmail.com

Background: Cosmetology is a science of beautifying skin and its appendages. Continuous conscious efforts of man towards looking beautiful as well as increasing skin problems caused by changing lifestyle have given birth to cosmetology. Its concept is present since Vedic times. Cosmetic approach is seen in Sushruta Samhita also. This is evident from the 22 upkramas explained under the heading of vaikritapaham as alepana, pariseka, utsadana, etc. Varnya dravya works on bhrajaka pitta, which is responsible for colour complexion. varnya dravyas helps in pitta, vata prasadana, ras, rakta shodhana thus improving varna. Objectives: To explore and compare clinically the varnya effect of Kumkumadi Taila and its cream preparations. Materials and Methods: A clinical study was conducted with 4 groups, containing 15 patients each. Patients were recruited from OPD of National Institute of Ayurveda, Jaipur. The following medicines were given for external application in the 4 groups. Group 1 received Kumkumadi Tailam, group 2 received varnya cream 1 (cold cream), group 3 received varnya cream 2 (vanishing cream) and group 4 received varnya cream 3 (all purpose cream) Results: It was found that Tailam therapy worked better than cream therapy. Conclusion: Cold cream worked better than all purpose cream, which in turn worked better than vanishing cream. Dark complexioned people got more benefit than fair complexioned.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/16: Evaluation of Ayurvedic herbal formulations

Pranav Shirkar, Neha Thali, Neha Khopkar, S.R. Kulkarni

Department of Pharmacognosy and Phytochemistry, Bombay

College of Pharmacy, Kalina, Santacruz (E), Mumbai, India

Email: shirkarpranav@gmail.com

Background: Ayurvedic herbal solid dosage forms namely pills and tablets were evaluated for stability of phytochemicals by HPTLC pattern and safety with respect to microbial bio-burden and pesticide residue. Objective: To study the safety and stability of herbal formulations prepared by conventional methods. Materials and Methods: The decoction of mixture of constituent herbs in proportions same as that of formulated pills and tablets were prepared. Pills were prepared manually by two methods namely sun drying and shade drying. The tablets were prepared by conventional wet granulation method using SLS as disintegrating agent. The granules were dried at 40°C and machine punched. HPTLC was performed to evaluate the stability of components in the formulation. The microbial bio-burden of formulations was studied by using Nutrient agar Hi-media make broth. Presence of pesticides like dimethoate, glyphosate and cypermethrin was tested by conventional TLC method. The mobile phase of dichloromethane: acetone (9:1) and water: ethanol: acetic acid (3.5:6:0.5) was used for dimethoate and glyphosate respectively which were visualized by spraying 0.3% ninhydrin. None of the formulation showed pesticide residue. Results: Microbial bio-burden results were as such, the pills did not show microbial growth whereas tablets showed 8.4 × 103 CFU/gm. Pesticide residue was found in none of the formulations. Conclusion: The above studies helped us in concluding that the herbal formulations prepared by conventional method are safe for use and have fairly good stability.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/17: Hypoglycaemic activity of Murrayakoenigii (meethaneem) leaves collected from Udaipur, Rajasthan

Pratibha Chaturvedi1, Abhay Chowdhary2

1Department of Biochemistry, Haffkine Institute for Training, Research and Testing, Parel, Mumbai, 2Director, Haffkine Institute for Training, Research and Testing, Parel, Mumbai, India

Email: pratibha.c@rediffmail.com

Background: Medicinal plants continue to provide treasured therapeutic agents by the ancient time. In many forms, plants are being used in the traditional systems of medicine in many parts of the world, especially in rural communities for diverse types of ailments. Diabetes mellitus is one of the utmost common chronic diseases in the whole world. Many synthetic drugs are used to control hyperglycaemia induced by Diabetes mellitus. However, owing to host resistance and other adverse effects of these compoundsthis becomesessential to find out the natural resource to fight with hyperglycaemia. ln current study, an approach was made to evaluate the hypoglycaemic activity of Murrayakoenigii (Meethaneem) leaves powder. Objective: Evaluation of hypoglycaemic activity of Murrayakoenigii (Meethaneem) leaves. Materials and Methods: Male wistar rat was used as experimental animal. Diabetes was induced by an intraperitoneal (i.p.) injection of streptozotocin. Animals with blood glucose >200mg % were selected and used for the study. Glibenclamide (500μg/kg; standard hypoglcaemic agent) and single dose of aqueous slurry of dried leaves powder of Murrayakoenigii were administered to selected animals. Blood was examined for its glucose level at 1-6, 24 hours separately. Results: Murrayakoenigii (Meethaneem) leaves slurry exhibited a significant hypoglycaemic activity on fourth day of dosing, which reduced the glucose level up to 56% that is more effective than standard drug Glebenclamide (49%). Conclusion: Murrayakoenigii (Meethaneem) leaves aqueous slurry has the potentiality to act as hypoglycaemic agent.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/18: Advance Technique in Treatment of Planter Fasciitis [Calcaneal Spur]

Ramesh Rajguru

Dr.Mane Medical Foundation and Research Centre, Rahuri, Ahmednagar, India

Email: drramesh12rajguru@gmail.com

Background: As mentioned in Ayurvediya Panchkarma chikitsa, Raktamokshan by using Leeches [Jalouka] is beneficial in the case of Planter Fasciitis. As Jaloukas are not easily available in the rural areas and if made available unable to survive in hot climatic conditions. So an alternate technique was developed using a 5ml syringe for the suction of blood. Materials and Methods: 5 ml syringe with cut from the nosal end and a hole made in the piston for the support to the syringe in proper direction, blade no 11, spirit, cotton, gauge, sticking, needles, gloves were used during the procedure. Patient lied on the examination table for the procedure. Painful area was located and marked with the pen. Small cuts known as Prachchan in Ayurveda were done with the help of blade, till bleeding starts from the superficial capillaries. Cuts should be up to 1mm deep. The syringe was applied over the bleeding area and 5ml blood was collected. Results: Patient felt complete relief after the procedure. Same procedure can be repeated after 15 days if there is partial relief. In most of cases there was no need of repetition. Conclusion: With the help of Raktamokshan by Prachchan karma, relief is observed in the patients of Planter Fasciitis.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/19: Role of Carica Papaya leaf extract on Dengue Fever and Dengue Haemorrhagic Fever

Jayashree C. Khendkar, J. J. Pawar, S. S. Sant

Department of Sharir Kriya, Government Ayurved College, Nanded, India Email: jayu914@yahoo.co.in

Dengue fever is a viral disease that caused by viral serotype Flaviviridae and carried by Aedes aegypti as the vector. Today dengue fever affects a vast number of people in over 125 countries and responsible for a number of deaths. A patient with dengue fever presents typically with fever, headache and rash known as the dengue triad. Dengue haemorrhagic fever and typically manifests as abdominal pain, bleeding, and even circulatory collapse. Infected patients receive supportive management with fluids, blood and blood products. There is still no specific antiviral drug available for the treatment of dengue fever. In Ayurveda, Erandkarkati i.e. Carica Papaya leaves has Swedjanan action also its prabhav is Jwaraghna. It is Raktashodhaka, Katupoushtika and Balya to Hridaya. Carica papaya from the family Caricaceae is a medicinal plant whose leaves contain carpinine and carpaine. The PTAFR gene which is known to be responsible for increased platelet production and aggregation was expressed 13.42-folds among the patients who consumed the papaya leaf juice as compared to the control group indicating that the juice had played an important role in arresting bleeding tendencies among these patients due to its immuno-modulatory effect. The administration of CPLJ (Carica papaya Leaf Juice) in dengue fever and dengue haemorrhagic fever is safe and does induce the rapid increase in platelet count. It may play a valuable role in the management of dengue fever in the near future.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/20: Study of correlation of Ama with free radical

Vilas S. Kad, Vrushali V Rane

SVNHT's Ayurved Mahavidyalaya, Shri Shivajinagar, Rahuri, Ahemadnagar, Maharashra, India

Email: dr.vrushalirane@yahoo.com

Ama is considered to be one of the important aspects of development of diseases process in the body. Majority of endogenous diseases beings with formation of ama. It has tremendous capacity to vitiate the doshas and disturbing the homestasis (dhatusamya). Ama is the resultant of improper digestion or partially digestion of the food particle due to hypofunction of jatharagni and also due to accumulation of mala in the body and also considered as parathamdoshdushti. It may be considered as partially or incompletely metabolized dhatu in case of dhatwagnimandya.In modern parameters, ama is supposed to be deadly free radical. Free radical is an atom/molecule that contain one or more unpaired electron, which requires neutralization by free radical scavengers. Ama is not a single entity but is a generalized term, which can be applied to many malformed substances in the body. This Ama is responsible for production of various diseases. In the same way free radical are also found to be the root cause of many diseases. Here we discus about properties and qualities of both entities. Which are similar and dissimilar and also whether free radical can be considered under Ama. The present article attempts to correlate the most recent concept of free radical with that of the concept of Ama with the help of some diseases like cancer, rheumatoid arthritis, stroke, mayocardial infraction, diabetes, alzheimer diseases, cardiovascular diseases, parkinson's diseases, atherosclerosis.

J Ayurveda Integr Med. 2015 May;6(Suppl 1):S26–S32.

UA/PP/21: In-vitro evaluation of Anti-oxidant and Anti-inflammatory activity of Triphala Churna, Dashmool Kadha, and Dashmool Bharad market formulations

Tejas V Mistry, Samidha G Joshi, Renuka P Munshi

Dept of Clinical Pharmacology, TNMC and BYL Nair Ch. Hospital, Mumbai, India

Email: tejas.v.mistry@gmail.com

Background: Triphala and Dashmool are 2 commonly used anti-inflammatory polyherbal formulations. Triphala is reported to have antioxidant, anti-inflammatory, antipyretic, analgesic, antibacterial, antimutagenic, anticancer, and hypoglycemic effects while Dashmool has anti-inflammatory properties and is effective in treating bronchitis, headache, cough, drowsiness, weakness, impotence, and digestive problems. Objective: The present study was carried out to evaluate the anti-oxidant and anti-inflammatory activities of Triphala churna, Dashmool bharad, and Dashmool kadha formulations using in-vitro methods. Materials and Methods: Triphala Churna (manufactured by Shree Dhootpapeshwar), Dashmool Kadha (manufactured by Sandhu brothers) and Dashmool Bharad (locally purchased) were used in the study. DPPH, ABTS and FRAC assays were used to evaluate the anti-oxidant properties while Protein denaturation and HRBC membrane stabilization assays were used evaluate anti-inflammatory activities of the formulations. α-Tocopherol was used as standard for anti-oxidant assay and Diclofenac sodium for anti-inflammatory assays. Results: 80μg of Triphala Churna showed 91.47% DPPH radical scavenging activity while α-Tocopherol showed 90.23%. 5mg of Dashmool Kadha showed 85.56% while Dashmool Bharad showed 27.19% DPPH radical scavenging activity. ABTS and FRAC showed similar results. All three formulations showed minimal anti-inflammatory activity in both the assays used. Conclusions: Triphala and Dashmool were found to have high antioxidant activity and radical scavenging activity against various antioxidant systems in vitro with minimal anti-inflammatory effect which needs to be confirmed by other assays.

Articles from Journal of Ayurveda and Integrative Medicine are provided here courtesy of Elsevier

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