Fig. 5.

COX-2 is expressed by neutrophils and urothelial cells in severely infected bladders. (A–B) Urinary bladder COX-1 (Ptgs1) and COX-2 (Ptgs2) gene expression was determined by qRT-PCR in (A) mock PBS-infected bladders and (B) 6 and 24 h after intravesical inoculation with either 10⁷ or 10⁸ cfu of the UPEC strain UTI89. (C) COX-2 gene expression at 24 hpi was detected in UPEC-infected bladder tissue by in situ hybridization. (D) COX-1, COX-2, and Ly6G protein expression at 24 hpi in UPEC-infected bladders was detected by immunofluorescence microscopy of paraffin sections. In panels C–D, bars approximate 50 μm, L indicates bladder lumen, U indicates urothelium, LP indicates lamina propria, and dashed line denotes the approximate location of the urothelial basement membrane. The arrow indicates the presence of an IBC in the section. See also Fig. S2.