NEUROSCIENCE Correction for “MeCP2 binds to non-CG methylated DNA as neurons mature, influencing transcription and the timing of onset for Rett syndrome,” by Lin Chen, Kaifu Chen, Laura A. Lavery, Steven Andrew Baker, Chad A. Shaw, Wei Li, and Huda Y. Zoghbi, which appeared in issue 17, April 28, 2015, of Proc Natl Acad Sci USA (112:5509–5514; first published April 13, 2015; 10.1073/pnas.1505909112).
The authors note that Fig. 2 appeared incorrectly. The corrected figure and its legend appear below.
Fig. 2.
MeCP2 binds the entire genome with differential affinity in the adult mouse brain. (A) Browser representation of mCG density and normalized MeCP2 binding spanning example chromosomal region (Top, representing the x axis). Shown are mCG density in the adult mouse brain (black), previously reported MeCP2 binding in ES cells (green), adult mouse brain (blue), and results from the current study in the adult mouse hypothalamus (red). (B) Correlation between MeCP2 binding profiles from our high-resolution data and previous data from the adult mouse brain (Upper) and ES cells (Lower). Raw reads were binned per 1 Mb across the entire mouse genome. r denotes Pearson’s correlation. (C) Enrichment of repetitive DNA elements from three biological replicates, showing significant increase of mouse major satellite DNA, but not L1 repetitive element after MeCP2 ChIP-Seq relative to Input DNA-Seq. Data were normalized and represented as the fold-change relative to each Input DNA-Seq control. (D) RT-PCR analysis of isolated DNA from ChIP using primers for two representative high-affinity sites (gray) and two low-affinity sites (white) in the mouse genome. Mouse major satellite DNA (black) was used as a positive control for the high-affinity site. Each bar represents SEM; n = 3 or 4; *P < 0.05 by one-way ANOVA followed by Student’s t test.