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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Virology. 2015 Mar 30;482:84–88. doi: 10.1016/j.virol.2015.03.007

Figure 4. AAV2 X helps rAAV/Foxp3 DNA and virion production driven by AAV6 rep/cap.

Figure 4

Here we demonstrate that AAV2 X also helps an AAV6-based rep/cap system. We used pRepCap6 which has both the AAV6 rep and cap genes. 293-X-B and 293-X-K are cell lines which contain the X gene. A shows a Southern blot of pAAV/Foxp3 DNA replication (probed with 32P-Foxp3 DNA.. B is a densitometric quantification of A. Note that in the presence of AAV2 X, included In 293-X-B and 293-X-K, the level of AAV/Foxp3 DNA replication was significantly higher compared to 293 cells. C shows a dot blot of DNaseI-resistant pAAV/Foxp3 virion DNA probed with 32P-Foxp3 DNA.. D is a densitometric quantification of C. Note that in the presence of AAV2 X, included In 293-X-B and 293-X-K, the level of AAV/Foxp3 virion DNA was significantly higher compared to 293 cells.