Fig. 7.

H₂O₂ injury induces sPLA₂-IIA expression in cultured oligodendrocytes. A) Western blots show a dose dependent increase in sPLA₂-IIA 48 hr following a 30 min injury with vehicle, 1mM, or 10mM of H₂O₂. B–J) Confocal images of oligodendrocytes 48 hr following treatment with vehicle (B-D) or 10 mM of H₂O₂ (E – J). Dead or dying cells showed either a fragmentation of the nuclei, loss of arborization, and micro-puncta (E – G) or a round nuclei, swelling of primary processes, and larger puncta (H – J). Bar: 50 μm.