Skip to main content
. 2015 Jun 9;6(3):e00392-15. doi: 10.1128/mBio.00392-15

FIG 7 .

FIG 7 

Characterization of genes involved in acetic acid-mediated biofilm formation. (A) Pellicle formation by the wild type (3610) and the ΔywcBA mutant (YC535) in MSgg and LBGM medium. Images were taken after 24 h of incubation at 30°C for LBGM medium or 48 h of incubation at 30°C for MSgg. (B and C). Assays of β-galactosidase activities of the wild-type cells and the ΔywcBA cells harboring either the PtapA-lacZ transcriptional reporter (B) or the PepsA-lacZ transcriptional reporter (C). (D) Assays of the β-galactosidase activities of the strains (FY250, FY251, and YC259) bearing one of the three promoter fusions (PyxaKC-lacZ, PysbAB-lacZ, and PywbHG-lacZ, respectively) for the three holin-antiholin operons. (E) Pellicle formation by the wild type, the three single mutants for the holin-antiholin-encoding genes (CY819 [ΔywbHG], CY881 [ΔysbAB], and CY882 [ΔyxaKC]), the triple mutant (CY886), and the strain overexpressing ywbHG (CY1250) inoculated in LBGM medium after 24 h of incubation at 30°C.