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PLOS ONE logoLink to PLOS ONE
. 2015 Jun 12;10(6):e0131261. doi: 10.1371/journal.pone.0131261

Correction: Hypothermia and Rewarming Induce Gene Expression and Multiplication of Cells in Healthy Rat Prostate Tissue

The PLOS ONE Staff
PMCID: PMC4466597  PMID: 26066324

The second sentence of the final paragraph of the Introduction is incorrect. The correct sentence is: Ventral lobes of rat prostate tissues were used in determination of the relative mRNA expressions of AMR, TM and the transmembrane form of the PAcP (Quintero et al. 2007). Cellular form of PAcP in the cytosol has been suggested to down-regulate prostate cell growth in human prostate cancer cells [15].

There are errors in Table 1, “Primers used in qPCR analysis.” Please see the corrected Table 1 here.

Table 1. Primers used in qPCR analysis.

Gene symbol/ accession number Direction Primer sequence Amplicon size
AMR NM_017123.1 Forward GTGCATGCCATTGCCTAGCTGA 78
Reverse TCATTTCCGGTGTGGCTTGGCA
Bax NM_017059.2 Forward CCAGGACGCATCCACCAAGAAGC 136
Reverse TGCCACACGGAAGAAGACCTCTCG
Bcl-2 NM_016993.1 Forward GAGGCTGGGATGCCTTTGTGGA 89
Reverse GCTGAGCAGCGTCTTCAGAGA
CyD1 NM_171992.4 Forward ATCAAGTGTGACCCGGACTG 216
Reverse GCCACTACTTGGTGACTCCC
HSF1 XM_006241890.1 Forward CCATGAAGCACGAGAACGAG 117
Reverse ACTGCACCAGTGAGATCAGGA
PAcP NM_001134901.1 Forward CGGGATCCTGGTGATATTGCT 70
Reverse CCGATACACGTCTCTCTGCC
p21 NM_080782.3 Forward ACATCTCAGGGCCGAAAACG 78
Reverse CTTGCAGAAGACCAATCGGC
TM NM_031771.2 Forward GATCTCCATTGCCAGCCT 140
Reverse CACGTGCTGCAGTACTACCT
GAPDH BC029618 Forward TGGAAGGACTCATGACCACA 160
Reverse TTCAGCTCAGGGATGACCTT

The legend for Fig 1, “Relative mRNA expressions of AMR, PAcP, TM, CyD1, p21 and HSF1 in rat ventral prostate,” does not appear. Please see the complete, correct Fig 1 legend here.

Fig 1. Relative mRNA expressions of AMR, PAcP, TM, CyD1, p21 and HSF1 in rat ventral prostate.

Fig 1

C, control; MH1, mild hypothermia 1; MH2, mild hypothermia 2; SH1, severe hypothermia 1; SH2, severe hypothermia 2; SHW1, severe hypothermia followed by rewarming at room temperature; SHW2, severe hypothermia followed by rewarming at +28C; **p≤0.01, ***p≤0.001 compared with MH1; ††p≤0.01, †††p≤0.001 compared with MH2; ‡‡p≤0.01, ‡‡‡p≤0.001 compared with SH1; data are mean ± SEM.

The legend for Fig 2, “Bax mRNA to Bcl-2 mRNA ratios in rat ventral prostate” does not appear. Please see the complete, correct Fig 2 caption here.

Fig 2. Bax mRNA to Bcl-2 mRNA ratios in rat ventral prostate.

Fig 2

C, control; MH1, mild hypothermia 1; MH2, mild hypothermia 2; SH1, severe hypothermia 1; SH2, severe hypothermia 2; SHW1, severe hypothermia followed by rewarming at room temperature; SHW2, severe hypothermia followed by rewarming at +28C; **p≤0.01 compared with MH1; †p≤0.05 compared with SHW1; data are mean ± SEM.

The legend for Fig 3, “EGFR-ligand AMR protein expression and proliferative Ki-67 index in rat ventral prostate” does not appear. Please see the complete, correct Fig 3 caption here. The publisher apologizes for these errors.

Fig 3. EGFR-ligand AMR protein expression and proliferative Ki-67 index in rat ventral prostate.

Fig 3

Mean AMR protein expression values in rat prostate ventral lobe tissues (A); cytosolic AMR immunoreactivity in SH1 (B) and in SHW1 (C); mean Ki-67 index values in rat prostate ventral lobe tissues (D); nuclear Ki-67 immunoreactivity in SH2 (E) and in SHW2 (F). C, control; MH1, mild hypothermia 1; MH2, mild hypothermia 2; SH1, severe hypothermia 1; SH2, severe hypothermia 2; SHW1, severe hypothermia followed by rewarming at room temperature; SHW2, severe hypothermia followed by rewarming at +28C; **p≤0.01, ***p≤0.001 compared with SHW1; ††p≤0.01, †††p≤0.001 compared with C; ‡p≤0.05 compared with MH2; scale bar = 50 μm; data are mean ± SEM.

Reference


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