Figure 4. Selective HDAC1,2 inhibition increases global chromatin-associated H3K27ac without altering H3K27me3 in EZH2 DLBCL cells.

Karpas-422 (A) and SUDHL4 (B) were treated with DMSO, 2μM ACY-957, 2μM ACY-1044, 0.5μM DZNEP, 0.5μM GSK126 or combinations of these drugs for 48h prior to chromatin extraction. Western blot analysis was done with anti-H3K27ac, H3K27me3 or EZH2 and histone H3 served as a loading control. LE and SE indicate long and short exposures, respectively.