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. 2015 Feb 5;6(8):5547–5566. doi: 10.18632/oncotarget.2587

Figure 2. Depletion of mutant p53 affects BRCA1 and RAD17 gene expression.

Figure 2

(A-C) Quantitative RT-PCR (qRT-PCR) analysis of BRCA1, RAD17 and CHK1 expression in SKBr3 (a), CAL27 (b) and MDA-MB-468 (c) cell lines transiently knocked-down for p53 expression with the indicated siRNA oligonucleotides. Standard deviation in each experiment was derived from the analysis of biological triplicates. (D-F) Western blot analysis was performed with 50 μg of whole cell extracts and probed with the indicated antibodies from si-p53 SKBr3 (d), sip53 CAL27 (e) and si-p53 sp MDA-MB-468 (f) and from si-GFP cells as control. Cells were grown for 48 h after transfection. (G-I) qRT-PCR analysis for BRCA1(g), RAD17 (h) and CHK1 (i) expression was carried out from H1299 cells transiently transfected for 48 h with 2 μg of pCDNA3-mutp53R175H. Western blotting analysis (J) from these cells was performed with 50 μg of whole cell extracts and probed with the indicated antibodies. The experiments were produced in biological triplicates. P-values of the shown qRT-PCR experiments were calculated with two-tailed t-test. Statistically significant results were with p-value < 0.05.