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. 2015 Feb 28;6(8):5978–5989. doi: 10.18632/oncotarget.3468

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Copyright: © 2015 Tiwana et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A) CFA of HeLa cells irradiated following 40 nM Ambion siRNA transfection with siNT, siTPK1, siTHTR1 and siTHTR1/siTPK1. Knockdown confirmed by qRT-PCR (inset) B) CFA of HeLa cells irradiated following 20 nM Ambion siRNA transfection with siNT and siTPC. siTPC knockdown confirmed by qRT-PCR (inset). Representative of three independent experiments, data points presented as mean +/− SD from triplicate wells, p-values generated by factorial 2-way ANOVA. Sensitization enhancement ratio at 0.10 surviving fraction (SER₁₀) calculated from non-linear regression of linear quadratic model. qRT-PCR: Unpaired two-sided students t-test comparing siNT to candidate knockdown t-tests*p<0.05, **p<0.01, ***p<0.001.