Figure 5. Fractionated irradiation treatment radiosensitizes HeLa cells after thiamine metabolism disruption.
A) Colony formation of HeLa cells irradiated with three single doses of 2 Gy provided on 3 consecutive days after 48 hrs transfection with 40 nM Ambion siRNA for siNT, siTPK1 and siTHTR1. B) Colony formation of HeLa cells irradiated with a three single doses of 2 Gy given on 3 consecutive days initiated after 72 hrs of incubation with DMSO (control) or 10 μM PyrH. Colony formation assay: representative of three independent experiments, data points presented as mean +/− SD from triplicate wells. Unpaired two-sided students t-tests comparing siNT/control to candidate knockdown/treatment **p<0.01, ***p<0.001.