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FXYD3 levels in MDA-MB-231 cells. MDA-MB-231 cells were treated for 48 h with PBS/0.1% ethanol control vehicle, 1 nM estrogen, 10 nM tamoxifen or with estrogen in combination with tamoxifen, and expression of FXYD3 was determined by flow cytometry using anti-FXYD3 antibodies and a Guava PC flow cytometer. Each bar represents the mean ± SD of FXYD3 fluorescence per cell for six samples obtained in separate experiments.
