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. 2015 Mar 2;2(4):334–348. doi: 10.18632/oncoscience.137

Figure 3. Effect of miR-148a expression on the invasive potential of Daoy and D283 cell lines.

Figure 3

Invasion potential was determined by studying migration through matrigel coated 8-μm pore size transwell inserts. The study was done using the stable polyclonal populations (P1, P2) of Daoy and D283 cells expressing miR-148a on doxycycline induction. (A) Representative images of the Calcein, AM labeled cells on the lower side of the transwell chamber membrane 36 h after seeding the indicated polyclonal populations of Daoy cells onto the transwell insert, with (d, e & f) or without (a, b & c) induction of miR-148a expression and after restoration of NRP1 expression (g, h) in doxycycline induced cells. (B-C) Y axis denotes the total fluorescence intensity of the invaded cells normalized to the total intensity of the initial cell number seeded of the indicated cell populations. (D) Y axis indicates the percent reduction in the fluorescence of the invaded cells of the indicated stable polyclonal populations on doxycycline induction (+DOX) of Daoy P1 and P2 population cells with (+NRP1) or without restoration of NRP1 expression. ** indicates p < 0.001; *** indicates p < 0.0001.