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. 1994 Sep 13;91(19):8842–8845. doi: 10.1073/pnas.91.19.8842

Kinetic intermediates in the reactions between peptides and proteins of major histocompatibility complex class II.

C Beeson 1, H M McConnell 1
PMCID: PMC44702  PMID: 8090733

Abstract

The kinetics of the reactions between fluorescently labeled sperm whale myoglobin-(110-121) peptide and the murine major histocompatibility complex class II protein I-Ed have been analyzed. The presence in solution of both short- and long-lived protein-peptide complexes is demonstrated by the biphasic dissociation of the myoglobin peptide from I-Ed. The formation of the long-lived terminal complex is preceded by a characteristic induction phase. It is shown that the initially formed complex of the myoglobin peptide and I-Ed is a kinetic intermediate that undergoes a unimolecular reaction to form the terminal complex. Reactions between peptides and the class II proteins thus involve an intermediate structurally distinct from the terminal complex. The terminal complex presumably has a structure that is biologically active and similar to the published class II protein-peptide crystal structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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