FIGURE 2.

A–C: (A) UV Strand displacement with matched Target and (B) THF containing mismatched Target and (C) discrimination between match and mismatch target strand. In this experiment a preformed duplex containing an equimolar mixture of 0.7 μM probe and 0.7 μM competitor (${\mathrm{C}}_6^{\ast }\mathrm{P}$-Black , ${\mathrm{C}}_{12}^{\ast }\mathrm{P}$–Red, ${\mathrm{C}}_{24}^{\ast }\mathrm{P}$ - Blue) is mixed with 0.7 μM target strand. As the temperature is raised from 0°C to 95°C, the curves obtained in the figures are obtained. In Figure 2A, the target is fully complementary with the probe. In Figure 2B, the target contains a single mismatch. In Figure 2C 0.7 μM of fully complementary and of mismatched targets are added simultaneously.