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. Author manuscript; available in PMC: 2015 Jun 17.

Published in final edited form as: Biopolymers. 2013 Jun;99(6):408–417. doi: 10.1002/bip.22213

Table I.

DSC Derived Thermodynamic Parameters for Probe-Target and Competitor-Probe Complexes^a

Probe-Target Complexes
	T_m (°C)	ΔH_cal(kcal mol⁻¹)	ΔS_cal (cal mol⁻¹ K⁻¹)^b	ΔC_p (cal mol⁻¹ K⁻¹)
PT	79.1 ± 0.3	172.2 ± 4.4	465.9 ± 23.3	1100 ± 220
PM	69.6 ± 0.3	155.5 ± 4.0	430.7 ± 21.5	800 ± 200
Competitor-Probe Complexes
	T_m (°C)	ΔH_cal(kcal mol⁻¹)	ΔS_cal (cal mol⁻¹ K⁻¹)^b	ΔC_p (cal mol⁻¹ K⁻¹)
$C_{6}^{*} P$	65.2 ± 0.3	153.5 ± 3.9	432.7 ± 21.6	1060 ± 210
$C_{12}^{*} P$	60.5 ± 0.3	147.3 ± 3.7	416.4 ± 20.8	1330 ± 260
$C_{24}^{*} P$	56.5 ± 0.3	140.8 ± 3.4	405.9 ± 20.3	1230 ± 250

^a

Strand Concentration C_t = 50 μM. Data are for the dissociation reaction.

^b

Standard state entropy is for a hypothetical 1M strand reference state.