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. 2015 Jun 18;3(3):e00101-15. doi: 10.1128/genomeA.00101-15

Genome Sequences of Six Paenibacillus larvae Siphoviridae Phages

Susan Carson a, Emily Bruff b, William DeFoor b, Jacob Dums b, Adam Groth b, Taylor Hatfield b, Aruna Iyer b, Kalyani Joshi b, Sarah McAdams b, Devon Miles b, Delanie Miller b, Abdoullah Oufkir b, Brinkley Raynor b, Sara Riley b, Shelby Roland b, Horace Rozier b, Sarah Talley b, Eric S Miller a,
PMCID: PMC4472882  PMID: 26089405

Abstract

Six sequenced and annotated genomes of Paenibacillus larvae phages isolated from the combs of American foulbrood-diseased beehives are 37 to 45 kbp and have approximately 42% G+C content and 60 to 74 protein-coding genes. Phage Lily is most divergent from Diva, Rani, Redbud, Shelly, and Sitara.

GENOME ANNOUNCEMENT

Phages were isolated from infected comb presented to the North Carolina Department of Agriculture from across the state. Phage isolation and propagation used Paenibacillus larvae host strains ATCC 9545 and ATCC 25747 grown on brain heart infusion (BHI) plus 0.4% glucose and 1 µg/ml thiamine in broth or agar at 30°C. Swabs were taken from infected comb cells and incubated in growth medium with selective bacteria for 24 to 48 h. Plaques were identified on top agar lawns of P. larvae and reisolated from plate streaks at least three times. Electron micrographs showed that all six phages have the Siphoviridae morphotype.

Confluent lysis on P. larvae-seeded top agar was used to prepare lysates from which DNA was extracted using the Promega DNA cleanup system. Genomic DNA was sequenced in the North Carolina State University (NCSU) Genomic Sciences Laboratory using fragmentation, sizing, adapter addition, and flow cell usage, as described (Illumina, Inc., San Diego, CA). Lily and Rani were sequenced by HiSeq 100-bp reads, with the remaining four genomes being sequenced using MiSeq 2 × 300-bp reads. CLC Genomics Workbench releases 2013 and 2014 were used for assembly. The physical ends of the Lily and Rani genomes were determined by ligation of genomic DNA, PCR amplification across the joined ends, and Sanger sequencing. All use cos packaging and are likely temperate phages. Lily has a 12-bp 5′ overhanging terminus (GGTGCGCGTGAG), and Rani has a 9-bp 3′ overhanging terminus (CGACTGCCC). Diva, Redbud, Shelly, and Sitara have physical genome ends like those of Rani, based on matching end nucleotides and similar sequence assembly patterns.

The genomes were annotated by students using DNA Master (http://cobamide2.bio.pitt.edu) on the NC State Virtual Computing Lab. Coding regions were predicted using Glimmer (1) and GeneMark (2), and start codons were chosen based on DNA Master ribosomal binding site (RBS) parameters. The absence of tRNAs was predicted using ARAGORN (3) and tRNAscan (4). Protein function and start sites were corroborated using NCBI BLASTp (5).

All six genomes encode two terminase subunits oriented at the 5′ end of the sequence, followed by genes for the tail and head that are generally syntenic. Rani and Redbud are nearly identical, and Lily is the most divergent. All contain genes typically seen in Siphoviridae phages (major capsid, portal, tape measure, tail, holin, endolysin, etc.). Most of the phages share regions of similarity with phiIBB_Pl23, a P. larvae Siphoviridae phage (6), and negligible similarity with the Myoviridae phages isolated from Utah (7). That all of the reported phages from Utah are Myoviridae and the North Carolina phages are Siphoviridae may reflect that the North Carolina phages were uniquely isolated from American foulbrood (AFB) diseased hives or that the Utah host used is substantially different from that of the P. larvae strains used in this work. Phage HB10c2 (GenBank accession no. KP202972), isolated from an AFB-diseased hive in Germany, has substantial sequence similarity to the genomes reported here.

Nucleotide sequence accession numbers.

The nucleotide sequence accession numbers are listed in Table 1.

TABLE 1 .

Characteristics of the phages in this study

Phage GenBank accession no. Strain host Coverage (×) Length (bp) G+C content (%) No. of genes
Diva KP296791 ATCC 9545 18,260 37,246 42.1 60
Lily KP296792 ATCC 9545 30,000 44,952 42.7 74
Rani KP296793 ATCC 9545 33,000 37,990 41.8 61
Redbud KP296794 ATCC 9545 14,801 37,971 41.8 61
Shelly KP296795 ATCC 9545 7,815 41,152 41.5 68
Sitara KP296796 ATCC 25747 6,516 43,724 41.6 74

ACKNOWLEDGMENTS

We thank G. Hackney of the North Carolina Department of Agriculture; C. Dashiell and J. Schaff of the NCSU Genomic Sciences Lab; Dan Russell (University of Pittsburgh) for helpful discussions; and the NCSU Biotechnology Program for support of Phage Hunters and Phage Genomics courses.

We also thank the HHMI SEA-PHAGES program for funding that launched the North Carolina State University Phage Hunters course and the Bayer Crop Science Bee Care Center for support and collaboration.

Footnotes

Citation Carson S, Bruff E, DeFoor W, Dums J, Groth A, Hatfield T, Iyer A, Joshi K, McAdams S, Miles D, Miller D, Oufkir A, Raynor B, Riley S, Roland S, Rozier H, Talley S, Miller ES. 2015. Genome sequences of six Paenibacillus larvae Siphoviridae phages. Genome Announc 3(3):e00101-15. doi:10.1128/genomeA.00101-15.

REFERENCES

  • 1.Delcher AL, Harmon D, Kasif S, White O, Salzberg SL. 1999. Improved microbial gene identification with Glimmer. Nucleic Acids Res 27:4636–4641. doi: 10.1093/nar/27.23.4636. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 2.Besemer J, Lomsadze A, Borodovsky M. 2001. GeneMarkS: a self-training method for prediction of gene starts in microbial genomes. Implications for finding sequence motifs in regulatory regions. Nucleic Acids Res 29:2607–2618. doi: 10.1093/nar/29.12.2607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 3.Schattner P, Brooks AN, Lowe TM. 2005. The tRNAscan-SE, snoscan and snoGPS Web servers for the detection of tRNAs and snoRNAs. Nucleic Acids Res 33:W686–W689. doi: 10.1093/nar/gki366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 4.Laslett D, Canback B. 2004. ARAGORN, a program to detect tRNA genes and tmRNA genes in nucleotide sequences. Nucleic Acids Res 32:11–16. doi: 10.1093/nar/gkh152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 5.Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. 1990. Basic local alignment search tool. J Mol Biol 215:403–410. [DOI] [PubMed] [Google Scholar]
  • 6.Oliveira A, Melo LD, Kropinski AM, Azeredo J. 2013. Complete genome sequence of the broad-host-range Paenibacillus larvae phage phiIBB_Pl23. Genome Announc 1(5):e00438-13. doi: 10.1128/genomeA.00438-13. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Sheflo MA, Gardner AV, Merrill BD, Fisher JN, Lunt BL, Breakwell DP, Grose JH, Burnett SH. 2013. Complete genome sequences of five Paenibacillus larvae bacteriophages. Genome Announc 1(6):e00668-13. doi: 10.1128/genomeA.00668-13. [DOI] [PMC free article] [PubMed] [Google Scholar]

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