Table 1.

List of oligonucleotide primers and TaqMan probe sequences used in this study

Name	Sequence	Product size
Primers used for Dab2ip methylation
CpG 131/MSP/M/F	5′-CGTTTTTTTTCGGTTTTAAACGTTTTTA-3′	243
CpG 131/MSP//M/R	5′-CGCCCTACAAACATCGTTCCCCGCGCGCT-3′	243
CpG 131/MSP /UM/F	5′-TGTTTTTTTTTGGTTTTAAATGTTTTTA-3′	243
CpG 131/MSP/UM/R	5′-CACCCTACAAACATCATTCCCCACACACT-3′	243
CpG 131/BSP/F	5′-GAATTTGGGGAATATGGAGTAGAATAG-3′	264
CpG 131/BSP/R	5′-TTCCCTACCTTCTTTACTATAACCCAA-3′	264
CpG 54/MSP/M/F	5′-TTAGGTTAGGATTTTGTTCGTATTGATTTTTC-3′	303
CpG 54/MSP/M/R	5′-CTCCCGATACTCTTCCTAACGTTACCGCCGAC-3′	303
CpG 54/MSP/UM/F	5′-TTAGGTTAGGATTTTGTTTGTATTGATTTTTT-3′	303
CpG 54/MSP/UM/R	5′-CTCCCAATACTCTTCCTAACATTACCACCAAC-3′	303
CpG 54/BSP/F	5′-GTTTAGATATGGTTGTTGGGTATATGTT-3′	243
CpG 54/BSP/R	5′-AAAACCAAAACTACCCTACAAAATAACT-3′	243
CpG 85/MSP/M/F	5′-GAGTTTTTCGTTGTTCGATATAAAAGGTATTTTC-3′	153
CpG 85/MSP/M/R	5′-CGAATCTTAAATTATACCCATTAACCGAACGCCT-3′	153
CpG 85/MSP/UM/F	5′-GAGTTTTTTGTTGTTTGATATAAAAGGTATTTTT-3′	153
CpG 85/MSP/UM/R	5′-CAAATCTTAAATTATACCCATTAACCAAACACCT-3′	153
CpG 85/BSP/F	5′-GAGGTGGGTATTGTTTTTTGAGTAG-3′	193
CpG 85/BSP/R	5′-TACTCCTCCCCTCCAAATATTC-3′	193
Primers used for Dab2ip RT-PCR
E1-53.84-F	5′-CGCTCATGGAGACGGCCTCGGTTCATAAATCA-3′
E7-117.149-R	5′-AGCAGTAGTCCTGACCCAGGATGCTGCTGTGAA-3′
Primers used for qRT-PCR
GRD domain-F	5′-GCCTTCTGCAAGATCATCAAC-3′
GRD domain-R	5′-GCTGATGAGCCGTTCACTG-3′
PH domain-F	5′-CGCGGACAATGAGAGGTC-3′
PH domain-R	5′-GAGCAGGGACTCGTGTGAC-3′

MSP, methyl specific primer; BSP, bisulfite specific primer; F, forward; R, reverse