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. 2015 Mar 8;4(3):409–422. doi: 10.1002/mbo3.248

Figure 1.

Figure 1

Production and purification of recombinant AhPDF1.1b in Pichia pastoris. (A) Analysis of the culture medium and the purified fraction of AhPDF1.1b produced in P. pastoris, on 18% (w/v) acrylamide ethylene-glycol SDS-PAGE. MW, molecular weight markers; Lane 1, culture medium, Coomassie staining. Lane 2, culture medium, western blot with anti-AhPDF1.1b polyclonal serum. Lane 3, final purified recombinant AhPDF1.1b, Coomassie staining. Lane 4, final purified recombinant AhPDF1.1b, western blot with anti-AhPDF1.1b polyclonal serum. (B) MALDI-TOF mass spectrum of the purified recombinant AhPDF1.1b produced in P. pastoris.