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. Author manuscript; available in PMC: 2016 Jul 17.
Published in final edited form as: AIDS. 2015 Jul 17;29(11):1287–1296. doi: 10.1097/QAD.0000000000000698

Figure 2. DCs incubated with sCD40L monomeric as compared to multimeric forms have differential effects on naïve CD4+ T cell skewing and proliferation.

Figure 2

DCs were incubated with sCD40L monomer (MONO) as compared to CD40L multimer (MULTI) or control DC (NEG) overnight and were then co-cultured with CFSE-labeled allogeneic naïve CD4 T cells where (A) % CFSE dilution is represented as % proliferation and (B) culture supernatants were assayed for IFNγ using the Human CBA. (C) sCD40L-stimulated DCs were subsequently activated with Poly-ICLC and co-cultured with allogeneic naïve CD4+ T cells. Shown in (A), (B), and (C), are data for three donors with statistical analysis using two-tailed Student's t tests. *Represents a p-value < 0.05 and was considered significant.