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Enzymatic ligation of the inactive 166mer and treatment with exonuclease RNase R. Lane 1: ligation mixture composed of ligation product and remaining nonligated linear transcript; lane 2: RNA size standard, 100 bases, 200 bases, 300 bases, 400 bases, 600 bases, 800 bases, and 1000 bases; lane 3: ligation product mixture after treatment with RNase R (for details see Supplemental Material).
