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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Hepatology. 2015 Feb 27;62(1):220–231. doi: 10.1002/hep.27677

Fig. 6. In vivo downregulation of LSD1 leads to impaired adaptive responses to a high BA load, resulting in increased hepatic BA levels, liver toxicity, and inflammation.

Fig. 6

(A) Experimental outline: Mice were injected with Ad-shRNA for LSD1 or control virus and 6 days later, the injected mice were fed normal chow or 0.5% CA chow for 6 days, and tissues and serum were collected. (B) Effects of downregulation of LSD1 in mice fed normal chow or 0.5% CA chow for 6 days on hepatic LSD1 protein levels detected by IB or IHC. (C, D) Effects of downregulation of LSD1 on gall bladder and BA pool sizes and liver and serum BA levels (C), on serum ALT/AST (D), and on liver histology detected by H&E staining (E). Abnormally accumulated bile is indicated by the arrows (E). (F) Effects of downregulation of LSD1 on infiltration of macrophages detected by IHC. (E, F) Mice were fed normal chow (-CA) or CA chow for 6 days (+CA). Arrows indicate infiltrated macrophages (F). The mean and SEM (n=3) are plotted and statistical significance was measured using the Student’s t-test. *p<0.05, **p<0.01 and NS statistically not significant.